Using a parallel-plate flow chamber, the hydrodynamic shear forces to prevent bacterial adhesion (Fprev) and to detach adhering bacteria (Fdet) were evaluated for hydrophilic glass, hydrophobic, dimethyldichlorosilane (DDS)-coated glass and six different bacterial strains, in order to test the following three hypotheses. 1. A strong hydrodynamic shear force to prevent adhesion relates to a strong hydrodynamic shear force to detach an adhering organism. 2. A weak hydrodynamic shear force to detach adhering bacteria implies that more bacteria will be stimulated to detach by passing an air–liquid interface (an air bubble) through the flow chamber. 3. DLVO (Derjaguin, Landau, Verwey, Overbeek) interactions determine the characteristic hydrodynamic shear forces to prevent adhesion and to detach adhering micro-organisms as well as the detachment induced by a passing air–liquid interface. Fprev varied from 0.03 to 0.70 pN, while Fdet varied from 0.31 to over 19.64 pN, suggesting that after initial contact, strengthening of the bond occurs. Generally, it was more difficult to detach bacteria from DDS-coated glass than from hydrophilic glass, which was confirmed by air bubble detachment studies. Calculated attractive forces based on the DLVO theory (FDLVO) towards the secondary interaction minimum were higher on glass than on DDS-coated glass. In general, all three hypotheses had to be rejected, showing that it is important to distinguish between forces acting parallel (hydrodynamic shear) and perpendicular (DLVO, air–liquid interface passages) to the substratum surface.
Abu-LailN. I.,
CamesanoT. A.2003; Role of lipopolysaccharides in the adhesion, retention, and transport of Escherichia coli JM109. Environ Sci Technol 37:2173–2183
AgladzeK.,
WangX.,
RomeoT.2005; Spatial periodicity of Escherichia coli K-12 biofilm microstructure initiates during a reversible, polar attachment phase of development and requires the polysaccharide adhesin PGA. J Bacteriol 187:8237–8246
AzeredoJ.,
VisserJ.,
OliveiraR.1999; Exopolymers in bacterial adhesion: interpretation in terms of DLVO and XDLVO theories. Colloids Surf B Biointerfaces 14:141–148
BakkerD. P.,
BusscherH. J.,
Van der MeiH. C.2002; Bacterial deposition in a parallel plate and a stagnation point flow chamber: microbial adhesion mechanisms depend on the mass transport conditions. Microbiology 148:597–603
BakkerD. P.,
PostmusB. R.,
BusscherH. J.,
Van der MeiH. C.2004; Bacterial strains isolated from different niches can exhibit different patterns of adhesion to substrata. Appl Environ Microbiol 70:3758–3760
BosR.,
Van der MeiH. C.,
BusscherH. J.1999; Physico-chemistry of initial microbial adhesive interactions – its mechanisms and methods for study. FEMS Microbiol Rev 23:179–230
BowenW. R.,
FentonA. S.,
LovittR. W.,
WrightC. J.2002; The measurement of Bacillus mycoides spore adhesion using atomic force microscopy, simple counting methods, and a spinning disk technique. Biotechnol Bioeng 79:170–179
BusalmenJ. P.,
de SanchezS. R.2001; Adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and polarized thin films of gold. Appl Environ Microbiol 67:3188–3194
CaoT.,
TangH. Y.,
LiangX. M.,
WangA. F.,
AunerG. W.,
SalleyS. O.,
NgK. Y. S.2006; Nanoscale investigation on adhesion of E. coli surface modified silicone using atomic force microscopy. Biotechnol Bioeng 94:167–176
DasS. K.,
SchechterR. S.,
SharmaM. M.1994; The role of surface-roughness and contact deformation on the hydrodynamic detachment of particles from surfaces. J Colloid Interface Sci 164:63–77
De KerchoveA. J.,
ElimelechM.2008; Calcium and magnesium cations enhance the adhesion of motile and nonmotile Pseudomonas aeruginosa on alginate films. Langmuir 24:3392–3399
DuddridgeJ. E.,
KentC. A.,
LawsJ. F.1982; Effect of surface shear-stress on the attachment of Pseudomonas fluorescens to stainless-steel under defined flow conditions. Biotechnol Bioeng 24:153–164
FallmanE.,
SchedinS.,
JassJ.,
AnderssonM.,
UhlinB. E.,
AxnerO.2004; Optical tweezers based force measurement system for quantitating binding interactions: system design and application for the study of bacterial adhesion. Biosens Bioelectron 19:1429–1437
Gomez-SuarezC.,
BusscherH. J.,
Van der MeiH. C.2001; Analysis of bacterial detachment from substratum surfaces by the passage of air–liquid interfaces. Appl Environ Microbiol 67:2531–2537
HigashiJ. M.,
WangI. W.,
ShlaesD. M.,
AndersonJ. M.,
MarchantR. E.1998; Adhesion of Staphylococcus epidermidis and transposon mutant strains to hydrophobic polyethylene. J Biomed Mater Res 39:341–350
JacobsA.,
LafolieF.,
HerryJ. M.,
DebrouxM.2007; Kinetic adhesion of bacterial cells to sand: cell surface properties and adhesion rate. Colloids Surf B Biointerfaces 59:35–45
KatsikogianniM.,
MissirlisY. F.2004; Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria–material interactions. Eur Cell Mater 8:37–57
MeindersJ. M.,
Van der MeiH. C.,
BusscherH. J.1995; Deposition efficiency and reversibility of bacterial adhesion under flow. J Colloid Interface Sci 176:329–341
Mendez-VilasA.,
Gallardo-MorenoA. M.,
Gonzalez-MartinM. L.2006; Nano-mechanical exploration of the surface and sub-surface of hydrated cells of Staphylococcus epidermidis
. Antonie Van Leeuwenhoek 89:373–386
MohamedN.,
TeetersM. A.,
PattiJ. M.,
HookM.,
RossJ. M.1999; Inhibition of Staphylococcus aureus adherence to collagen under dynamic conditions. Infect Immun 67:589–594
MohamedN.,
RainierT. R.,
RossJ. M.2000; Novel experimental study of receptor-mediated bacterial adhesion under the influence of fluid shear. Biotechnol Bioeng 68:628–636
OwensN. F.,
GingellD.,
RutterP. R.1987; Inhibition of cell-adhesion by a synthetic-polymer adsorbed to glass shown under defined hydrodynamic stress. J Cell Sci 87:667–675
RoosjenA.,
BoksN. P.,
Van der MeiH. C.,
BusscherH. J.,
NordeW.2005; Influence of shear on microbial adhesion to PEO-brushes and glass by convective-diffusion and sedimentation in a parallel plate flow chamber. Colloids Surf B Biointerfaces 46:1–6
RutterP. R.,
VincentB.1988; Attachment mechanisms in the surface growth of microorganisms. In Physiological Models in Microbiology pp 87–107 Edited by
BazinM. J.,
ProsserJ. I.
Boca Raton, FL: CRC Press;
SharmaP. K.,
RaoK. H.2003; Adhesion of Paenibacillus polymyxa on chalcopyrite and pyrite: surface thermodynamics and extended DLVO theory. Colloids Surf B Biointerfaces 29:21–38
ShiveM. S.,
HasanS. M.,
AndersonJ. M.1999; Shear stress effects on bacterial adhesion, leukocyte adhesion, and leukocyte oxidative capacity on a polyetherurethane. J Biomed Mater Res 46:511–519
SimpsonK. H.,
BowdenM. G.,
HookM.,
AnvariB.2002; Measurement of adhesive forces between S. epidermidis and fibronectin-coated surfaces using optical tweezers. Lasers Surg Med 31:45–52
SimpsonK. H.,
BowdenA. G.,
PeacockS. J.,
AryaM.,
HookM.,
AnvariB.2004; Adherence of Staphylococcus aureus fibronectin binding protein A mutants: an investigation using optical tweezers. Biomol Eng 21:105–111
Vadillo-RodriguezV.,
BusscherH. J.,
NordeW.,
De VriesJ.,
Van der MeiH. C.2004; Atomic force microscopic corroboration of bond aging for adhesion of Streptococcus thermophilus to solid substrata. J Colloid Interface Sci 278:251–254
Van HoldeK. E.1971; Introduction in transport processes: diffusion. In Physical Biochemistry, 1st edn. pp 79–111 Edited by
HoldeK. E. Van.
Englewood Cliffs, NJ: Prentice-Hall;
Van OssC. J.1994b; Relation between the Hamaker constant and the apolar surface tension component. In Interfacial Forces in Aqueous Media pp 154–160 Edited by
Van OssC. J.
New York: Marcel Dekker;
Van OssC. J.,
GoodR. J.,
ChaudhuryM.1986; The role of van der Waals forces and hydrogen bonds in hydrophobic interactions between biopolymers and low energy surfaces. J Colloid Interface Sci 111:378–390
WalkerS. L.,
RedmanJ. A.,
ElimelechM.2004; Role of cell surface lipopolysaccharides in Escherichia coli K12 adhesion and transport. Langmuir 20:7736–7746
WangI. W.,
AndersonJ. M.,
JacobsM. R.,
MarchantR. E.1995; Adhesion of Staphylococcus epidermidis to biomedical polymers – contributions of surface thermodynamics and hemodynamic shear conditions. J Biomed Mater Res 29:485–493