1887

Abstract

Our newly designed primers were evaluated for the molecular analysis of specific groups of the gene encoding sulfide : quinone reductase (SQR) in sediment environments. Based on the phylogenetic analysis, we classified the sequences into six groups. PCR primers specific for each group were developed. We successfully amplified -like gene sequences related to groups 1, 2 and 4 from diverse sediments including a marine sediment (SW), a tidal flat (TS), a river sediment (RS) and a lake sediment (FW). We recovered a total of 82 unique phylotypes (based on a 95 % amino acid sequence similarity cutoff) from 243 individual -like gene sequences. Phylotype richness varied widely among the groups of -like gene sequences (group 1>group 2>group 4) and sediments (SW>TS>RS>FW). Most of the -like gene sequences were affiliated with the clade and were distantly related to the reference gene sequences from cultivated strains (less than ∼80 % amino acid sequence similarity). Unique -like gene sequences were associated with individual sediment samples in groups 1 and 2. This molecular tool has also enabled us to detect -like genes in a sulfur-oxidizing enrichment from marine sediments. Collectively, our results support the presence of previously unrecognized gene-containing micro-organisms that play important roles in the global biogeochemical cycle of sulfur.

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2008-10-01
2020-03-31
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