1887

Abstract

In this study, the gene encoding the leucine-responsive regulatory protein (Lrp) in serovar Typhimurium was found to be negatively autoregulated. Its transcription start site was determined by primer extension analysis, showing that the promoter is located at a different site to that inferred previously from the Typhimurium genome sequence. Chromosomal DNA fragments that include the promoter region were bound by purified Lrp protein , producing up to four distinct protein–DNA complexes. DNase I footprinting identified regions that were protected by the protein as well as bases that became hypersensitive to DNase I treatment following Lrp binding. A clear pattern of periodic hypersensitivity was detected between positions −130 and +15 that was consistent with wrapping of the DNA around Lrp in a nucleoprotein complex that includes the putative promoter region. Lrp–DNA interaction in this region was fully consistent with the observed repression of transcription by this protein. Leucine was found to modulate Lrp-mediated autorepression by remodelling the Lrp–DNA nucleoprotein complex.

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2008-07-01
2020-08-15
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