1887

Abstract

-Oxidative enzymes for fatty acid degradation (Fad) of long-chain fatty acids (LCFAs) are induced during lung infection in cystic fibrosis patients, and this may contribute to nutrient acquisition and pathogenesis of . The promoter region of one -oxidation operon, (PA3014 and PA3013), was mapped. Focusing on the transposon mutagenesis of strain PAO1 carrying the P fusion, a regulator for the operon was identified to be PsrA (PA3006). Transcriptome analysis of the Δ mutant indicated its importance in regulating -oxidative enzymes. These microarray data were confirmed by real-time RT-PCR analyses of the and (encoding a lipase) genes. Induction of the operon was demonstrated to respond to novel LCFA signals, and this induction required the presence of PsrA, suggesting that LCFAs bind to PsrA to derepress . Electrophoretic mobility shift assays indicate specific binding of PsrA to the promoter region. This binding is disrupted by specific LCFAs (C , C, C and, to a lesser extent, C), but not by other medium- or short-chain fatty acids or the first intermediate of -oxidation, acyl-CoA. It is shown here that PsrA is a regulator that binds and responds to LCFA signals in .

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2008-06-01
2020-01-22
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