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Volume 154, Issue 7

Cellulose modulates biofilm formation by counteracting curli-mediated colonization of solid surfaces in Free

Abstract

In enterobacteria, the CsgD protein activates production of two extracellular structures: thin aggregative fimbriae (curli) and cellulose. While curli fibres promote biofilm formation and cell aggregation, the evidence for a direct role of cellulose as an additional determinant for biofilm formation is not as straightforward. The MG1655 laboratory strain of only produces limited amounts of curli and cellulose; however, ectopic expression results in strong stimulation of curli and cellulose production. We show that, in a -overexpressing derivative of MG1655, cellulose production negatively affects curli-mediated surface adhesion and cell aggregation, thus acting as a negative determinant for biofilm formation. Consistent with this observation, deletion of the gene, necessary for cellulose production, resulted in a significant increase in curli-dependent adhesion. We found that cellulose production increased tolerance to desiccation, suggesting that the function of cellulose might be related to resistance to environmental stresses rather than to biofilm formation. Production of the curli/cellulose network in enterobacteria typically takes place at low growth temperature (<32 °C), but not at 37 °C. We show that CsgD overexpression can overcome temperature-dependent control of the curli-encoding operon, but not of the cellulose-related gene, suggesting very tight temperature control of cellulose production in MG1655.

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Keyword(s): CV, crystal violet
SGM
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2008-07-01
2024-04-23
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Cellulose modulates biofilm formation by counteracting curli-mediated colonization of solid surfaces in Escherichia coli
Microbiology 154, 2017 (2008); https://doi.org/10.1099/mic.0.2008/018093-0
/content/journal/micro/10.1099/mic.0.2008/018093-0
/content/journal/micro/10.1099/mic.0.2008/018093-0
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