1887

Abstract

LysR-type transcriptional regulators (LTTRs) are one of the key players that help bacteria adapt to different environments. We have designated STM0952, a putative LTTR in serovar Typhimurium ( Typhimurium), as hydrogen peroxide resistance gene (). A knockout mutant of Typhimurium was sensitive to oxidative products of the respiratory burst, specifically to HO. The mutant is profoundly attenuated in a murine model of infection and showed decreased intracellular proliferation in macrophages. It also induced increased amounts of reactive oxygen species and co-localization with gp91phox in the macrophage cell line, when compared to the wild-type. A strain overexpressing the gene showed a survival advantage over the wild-type under HO-induced stress. Microarray analysis suggested the presence of an Hrg regulon, which is required for resistance to the toxic oxidative products of the reticuloendothelial system.

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2008-09-01
2020-07-16
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