1887

Abstract

-Mannosidases play an important role in the processing of mannose-containing glycans in eukaryotes. A deficiency in -mannosidase is lethal in humans and cattle. In contrast to mammals, does not require the endoplasmic reticulum -mannosidase gene for growth. However, little is known of the consequence of loss of function of class I -mannosidases in filamentous fungi. In this study, the /Af gene was identified to encode 1,2--mannosidase MsdS in . Soluble MsdS expressed in was characterized as a typical class I -mannosidase. The gene was deleted by replacement of the gene with a gene. Although the mutant showed a defect in -glycan processing, as well as a reduction of cell wall components and a reduced ability of conidiation, it appeared that the rate of hyphal growth was not affected. Morphology analysis revealed abnormal polarity and septation at the stages of germination, hyphal growth and conidiation. Although the mechanism by which the -glycan processing affects polarity and septation is unclear, our results show that is involved in polarity and septation in .

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2008-07-01
2020-04-04
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