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Volume 154, Issue 6

Analysis of the outer-membrane subproteome and identification of new antigenic targets for vaccine by immunomics Free

Abstract

is an important infectious Gram-negative bacterium causing cold-water disease (CWD) and rainbow trout fry syndrome. Outer-membrane proteins (OMPs) are key molecules with regard to the interface between the cell and its environment. Therefore, we sought to define the outer-membrane (OM) subproteome of in order to gain insight into the biology and pathogenesis of this bacterium and to identify the dominant antigens targeted by the rainbow trout () immune system during infection. First, OMs were prepared from a cell-envelope suspension by differential Sarkosyl (sodium lauryl sarcosinate) solubility. We then isolated the OMPs and identified 36 proteins from 34 spots resolved by two-dimensional electrophoresis and LC-MS/MS. An immunoproteomic approach using antibodies from CWD-convalescent rainbow trout was then used to identify 25 immunoreactive antigens that may be relevant in pathogenesis and diagnosis. These included the previously characterized surface-exposed OMPs OmpA, OmpH/P18 and FspA, as well as newly described antigenic proteins. This study provides a number of novel candidate proteins for developing vaccine(s) against flavobacteriosis infection in aquaculture.

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  • Accepted:
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  • Published Online:
Keyword(s): ACN, acetonitrile , ASB-14, 3-[N,N-dimethyl(3-myristoylaminopropyl)ammonio]propanesulfonate , CWD, cold-water disease , GRAVY, grand average of hydropathy , OM, outer membrane , OMP, outer-membrane protein and Sarkosyl, sodium lauryl sarcosinate
SGM
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2008-06-01
2024-04-19
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Analysis of the Flavobacterium psychrophilum outer-membrane subproteome and identification of new antigenic targets for vaccine by immunomics
Microbiology 154, 1793 (2008); https://doi.org/10.1099/mic.0.2008/016600-0
/content/journal/micro/10.1099/mic.0.2008/016600-0
/content/journal/micro/10.1099/mic.0.2008/016600-0
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