1887

Microbiology

Volume 154, Issue 6

heat-shock proteins IbpA/B are involved in resistance to oxidative stress induced by copper Free

Abstract

The small heat-shock proteins IbpA/B are molecular chaperones that bind denatured proteins and facilitate their subsequent refolding by the ATP-dependent chaperones DnaK, DnaJ, GrpE and ClpB. In this report, we demonstrate that IbpA/B participate in the defence against copper-induced stress under aerobic conditions. In the presence of oxygen, Δ cells exhibit increased sensitivity to copper ions and accumulate elevated amounts of oxidized proteins, while under oxygen depletion, the Δ mutation has no effect on copper tolerance. This indicates that IbpA/B protect cells from oxidative damage caused by copper. We show that AdhE, one of the proteins exposed to oxidation, is protected by IbpA/B against copper-mediated inactivation both and .

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): DNP, 2,4-dinitrophenol , DNPH, 2,4-dinitrophenylhydrazine , Hsp, heat-shock protein , MCO, metal-catalysed oxidation , ROS, reactive oxygen species and sHsp, small heat-shock protein
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2008-06-01
2019-10-15
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Escherichia coli heat-shock proteins IbpA/B are involved in resistance to oxidative stress induced by copper
Microbiology 154, 1739 (2008); https://doi.org/10.1099/mic.0.2007/014696-0
/content/journal/micro/10.1099/mic.0.2007/014696-0
/content/journal/micro/10.1099/mic.0.2007/014696-0
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