1887

Abstract

Genome sequencing of complex members has accelerated the search for new disease-control tools. Antigen mining is one area that has benefited enormously from access to genome data. As part of an ongoing antigen mining programme, we screened genes that were previously identified by transcriptome analysis as upregulated in response to an acid shock for their expression profile and antigenicity. We show that the genes encoding two methyltransferases, and , were highly upregulated in a mouse model of infection, and were antigenic in -infected cattle. As the genes encoding these antigens were highly upregulated , we sought to define their genetic regulation. A mutant was constructed that was deleted for their putative regulator, ; loss of the regulator led to increased expression of the flanking methyltransferases and a defined set of distal genes. This work has therefore generated both applied and fundamental outputs, with the description of novel mycobacterial antigens that can now be moved into field trials, but also with the description of a regulatory network that is responsive to both and stimuli.

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2008-04-01
2019-11-19
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Primers used in real-time qRT-PCR reactions [ PDF] (15 kb) Primers used in plasmid construction [ PDF] (10 kb)

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Primers used in real-time qRT-PCR reactions [ PDF] (15 kb) Primers used in plasmid construction [ PDF] (10 kb)

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