1887

Abstract

AtrA, a transcriptional activator for II-ORF4, encoding the pathway-specific transcriptional activator of the actinorhodin biosynthetic gene cluster in A3(2), has been shown to bind the region upstream from the promoter of , encoding the pathway-specific transcriptional activator of the streptomycin biosynthetic gene cluster in [Uguru (2005) , 131–150]. The orthologue () in was constitutively transcribed throughout growth from a promoter located about 250 nt upstream of the translational start codon, as determined by S1 nuclease mapping. DNase I footprinting showed that histidine-tagged AtrA-g bound an inverted repeat located upstream of at positions –117 to –142 relative to the transcriptional start point of as +1. This AtrA-g-binding site was between two AdpA-binding sites at approximately nucleotide positions –270 and –50. AdpA is a central transcriptional activator in the A-factor regulatory cascade and essential for the transcription of . AtrA-g and AdpA simultaneously bound the respective binding sites. In contrast to AdpA, AtrA-g was non-essential for transcription; an -disrupted strain produced streptomycin on routine agar media to the same extent as the wild-type strain. However, the -disrupted strain tended to produce a smaller amount of streptomycin than the wild-type strain under some conditions, for example, on Bennett agar containing 1 % maltose and on a minimal medium. Therefore, AtrA-g had a conditionally positive effect on streptomycin production, as a tuner, probably by enhancing the AdpA-dependent transcriptional activation of in a still unknown manner.

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2008-03-01
2021-07-30
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