1887

Abstract

The Cg1547 protein of ATCC 13032 is a member of the LacI/GalR family of DNA-binding transcriptional regulators. A defined deletion in the gene, now designated (uridine utilization regulator), resulted in the mutant strain KB1547. Comparison of gene expression levels in KB1547 and the wild-type strain revealed enhanced expression of the operon genes (ribokinase), (uridine transporter) and (uridine-preferring nucleoside hydrolase). Gene expression of the operon was stimulated by the presence of either uridine or ribose. Growth assays with mutants showed that functional Cg1543 and Cg1545 proteins are essential for the utilization of uridine as the sole carbon source. Transcriptional regulation of the operon is mediated by a 29 bp palindromic sequence composed of two catabolite-responsive element ()-like sequences and located in between the mapped −10 promoter region and the start codon of . A similar sequence was detected in the upstream region of (), coding for a second ribokinase in ATCC 13032. DNA band-shift assays with a streptavidin-tagged UriR protein and labelled oligonucleotides including the -like sequences of and demonstrated the specific binding of the purified regulator . Whole-genome DNA microarray hybridizations comparing the gene expression in KB1547 with that of the wild-type strain revealed that UriR is a pathway-specific repressor of genes involved in uridine utilization in .

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2008-04-01
2024-12-13
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