1887

Abstract

We have previously shown that copper uptake and regulation in the opportunistic pathogen has some similarities to those in , including the activation of the copper transporter gene under low-copper conditions by the transcription factor CaMac1p. However, in this study, further analysis has shown that the actual mechanism of regulation by CaMac1p is different from that of its homologue. We demonstrate for the first time, to our knowledge, that the gene is transcriptionally autoregulated in a copper-dependent manner, in contrast to , which is constitutively transcribed. We also demonstrate that the presence of one copper response element in the promoters of , and the ferric/cupric reductase gene is sufficient for normal levels of copper-responsive transcription. In contrast, two promoter elements are essential for normal levels of copper-dependent transcriptional activation by ScMac1p. CaMac1p is also involved in the regulation of the iron-responsive transcriptional repressor gene and the alternative oxidase gene . This work describes a key feature of the copper uptake system in that distinguishes it from similar processes in the model yeast . The importance of copper uptake in the environment of the human host and the implications for the disease process are discussed.

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2008-05-01
2019-11-22
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