The acidic, glutamine-rich Mpn474 protein of is surface exposed and covers the complete cell Free

Abstract

The protein Mpn474 encoded by the gene of the human-pathogenic contains 1033 amino acids and has an isoelectric point of 4.79, which is caused by the large excess of glutamic acid residues (11 %). Although the protein lacks recognizable export signals we showed by immuno-electron microscopy that Mpn474 is surface exposed, covering the cell completely. By combining cross-linking and careful treatment of the bacterial cells with Triton X-100, we found that this protein is weakly bound to the cell surface, while the true transmembrane protein Mpn141 (adhesin P1) is firmly attached under the same experimental conditions. A transposon mutant in the gene which has no obvious phenotype, served as negative control for the immunodetection.

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2008-04-01
2024-03-29
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