1887

Abstract

An insertion sequence, IS, of 1351 bp has been isolated from . Formation of IS circles containing a 3 bp spacer (complete junction) or deletion of 25 bp at the left inverted repeat (IRL) between the abutted IS ends of the IS junction region (deleted junction) was also discovered in the lactobacilli and system studied. We found that the promoter formed by the complete junction P was more active than that formed by the 25 bp deleted junction P or the indigenous promoter P. The corresponding transcription start sites for both promoter P and P as well as P were subsequently determined using a primer extension assay. The activity of transposase OrfAB of IS was also assayed using an system. It was found that this transposase preferred to cleave a single DNA strand at the IRR over the IRL end in the transposition process, suggesting that attack of one end by the other was oriented from IRR to IRL.

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2008-04-01
2020-03-29
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