1887

Abstract

Adherent and invasive mucosa-associated have been implicated in the pathogenesis of colon cancer and inflammatory bowel diseases. It has been reported that such isolates share features of extraintestinal (ExPEC) and particularly uropathogenic (UPEC). We used suppression subtractive hybridization (SSH) to subtract the genome of K-12 from that of a colon cancer mucosal isolate. Of the subtracted sequences, 53 % were present in the genomes of one or more of three sequenced UPEC strains but absent from the genome of an enterohaemorrhagic (EHEC) strain. Of the subtracted sequences, 80 % matched at least one UPEC genome, whereas only 4 % were absent from the UPEC genomes but present in the genome of the EHEC strain. A further genomic subtraction against the UPEC strain 536 enriched for sequences matching mobile genetic elements, other ExPEC strains, and other UPEC strains or commensals, rather than strains associated with gastrointestinal disease. We analysed the distribution of selected subtracted sequences and UPEC-associated pathogenicity islands (PAIs) amongst a panel of mucosa-associated isolated from colonoscopic biopsies of patients with colon cancer, patients with Crohn's disease and controls. This enabled us to identify a group of isolates from colon cancer (30–40 %) carrying multiple genes previously categorized as UPEC-specific and implicated in virulence.

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2008-02-01
2019-11-21
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Supplements

vol. , part 2, pp. 571 - 583

Supplementary Tables S1-S3.

isolates used in this study and distribution of sequences according to PCR assays

Oligonucleotide primers used for PCR amplification

Percentage distribution of PAIs amongst collections

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