1887

Abstract

In this study, we demonstrate that , an alternative factor of , is involved in cell surface stresses. Cells in which the gene was deleted evidenced increased sensitivity to magnesium deficiency, as well as to SDS, lysozymes, EDTA and heat. We utilized physiological analyses to show that the downstream gene, designated , encodes an anti- factor. The retarded growth of the mutant cells under ordinary growth conditions could be recovered by an additional deletion of encoding . Under stress conditions, the phenotype of the -overexpressing cells mimicked that of the mutant. The and genes were transcribed into a single transcript, and gene transcription was stimulated by heat. The SigE and CseE proteins interacted physically in the form of glutathione -transferase (GST) and maltose binding protein (MBP) fusion proteins, respectively. 2D-PAGE analysis of the wild-type and mutant crude extracts showed that the mutant failed to synthesize a 34 kDa polypeptide that was normally induced in wild-type cells grown under heat (or SDS)-stressed conditions. The protein turned out to be expressed from ORF NCgl1070 and showed similarity to methyltransferases which may confer resistance to antibiotics. Accordingly, the mutant evidenced extreme sensitivity to antibiotics, including nalidixic acid, penicillin and vancomycin. Finally, we present a discussion of the possible role of the and genes in the acclimation of to cell surface stress conditions.

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2008-03-01
2019-10-20
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