1887

Abstract

The specific enzymes employed by for the anaerobic oxidation of thiosulfate, sulfide and elemental sulfur during anoxygenic photosynthesis are not well defined. In particular, it is unclear how completely oxidizes thiosulfate. A genomic region, encoding a putative quinone-interacting membrane-bound oxidoreductase (Qmo) complex (), hypothetical proteins () and a sulfide : quinone oxidoreductase (SQR) homologue (), was analysed for its role in anaerobic sulfur oxidation. Transcripts of genes encoding the Qmo complex, which is similar to archaeal heterodisulfide reductases, were detected by RT-PCR only while sulfide or elemental sulfur were being oxidized, whereas the SQR homologue and were expressed during thiosulfate oxidation and into early stationary phase. A mutant of was obtained in which the region between and was replaced by a transposon insertion resulting in the truncation or deletion of nine genes. This strain, C5, was completely defective for growth on thiosulfate as the sole electron donor in , but only slightly defective for growth on sulfide or thiosulfate plus sulfide. Strain C5 did not oxidize thiosulfate and also displayed a defect in acetate assimilation under all growth conditions. A gene of unknown function, , deleted in strain C5 that is conserved in chemolithotrophic sulfur-oxidizing bacteria and archaea is the most likely candidate for the thiosulfate oxidation phenotype observed in this strain. The defect in acetate assimilation may be explained by deletion of , which encodes a homologue of 3-oxoacyl acyl carrier protein synthase.

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2008-03-01
2019-11-12
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Distribution of orthologues (best bidirectional BLASTP matches) to the proteins encoded by CT0869 and CT0872 in other complete and draft genome sequences [ Excel file] (22 kb) Proteins containing a single ACP synthase III domain as in CT0874 [ Excel file] (21 kb)

EXCEL

Distribution of orthologues (best bidirectional BLASTP matches) to the proteins encoded by CT0869 and CT0872 in other complete and draft genome sequences [ Excel file] (22 kb) Proteins containing a single ACP synthase III domain as in CT0874 [ Excel file] (21 kb)

EXCEL
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