1887

Abstract

is found in biofilms on teeth and as a commensal member of the gastrointestinal and urinary floras, but may also be associated with deep-seated purulent infections and infective endocarditis. produces hyaluronidase, an enzyme that breaks down hyaluronan (HA), a major component of the extracellular matrix of connective tissue. We investigated the involvement of hyaluronidase in biofilm formation and dispersal as well as adhesion to human cells. The hyaluronidase activity and expression of the gene were higher in growth media supplemented with HA. Inactivation of the hyaluronidase resulted in a mutant that formed up to 31 % more biofilm in media supplemented with HA. Hyaluronidase added to the medium caused dispersal of biofilm. Adhesion to epithelial cells was similar in the wild-type and the hyaluronidase mutant. We concluded that hyaluronidase may be important for detachment from biofilms but not for adhesion to epithelial cells. The ability of to detach from the surface and to spread may be crucial in the pathogenicity of this micro-organism.

Keyword(s): HA, hyaluronan
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2008-03-01
2019-10-13
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