1887

Abstract

This paper describes an investigation of the complex internal regulatory circuitry of the staphylococcal locus and the impact of modifying this circuitry on the expression of external genes in the regulon. The locus contains four genes, the and two-component signalling module (TCS), and and two upstream genes of hitherto unknown function. It is expressed from two promoters, P , which transcribes only the TCS, and P , which transcribes the entire locus. A (bursa) transposon insertion in in a derivative of NCTC 8325 has a profound effect on function. It modifies the activity of the TCS, changing the expression of many genes in the regulon, even though transcription of the TCS (from P ) is not interrupted. Moreover, these effects are not due to disruption of since an in-frame deletion in has essentially no phenotype. The phenotype of strain Newman is remarkably similar to that of the  : : bursa and this similarity is explained by an amino acid substitution in the Newman gene that is predicted to modify profoundly the signalling function of the protein. This concurrence suggests that the  : : bursa insertion affects the signalling function of , a suggestion that is supported by the ability of an clone, but not an clone, to complement the effects of the  : : bursa insertion.

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2008-03-01
2020-08-12
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