1887

Abstract

is a Gram-negative facultative intracellular pathogen, which multiplies in protozoa in its natural environment and can cause Legionnaires' disease in man, following infection of alveolar macrophages. In each of the different stages of infection of host cells, virulence proteins need to be delivered to their specific place of action and therefore must cross two barriers: the inner and the outer membrane. To date, several specialized secretion machineries for transport of proteins across the inner and outer membrane have been identified in . Most of these secretion pathways have been shown to affect the virulence of this pathogen. An overview will be given of all the secretion pathways and the proteins transported by these secretion systems identified so far, with special attention paid to those that play a role in the pathogenicity of .

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2007-12-01
2024-12-03
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References

  1. Albert-Weissenberger C., Cazalet C., Buchrieser C. 2007; Legionella pneumophila – a human pathogen that co-evolved with fresh water protozoa. Cell Mol Life Sci 64:432–448
    [Google Scholar]
  2. Bandyopadhyay P., Liu S., Gabbai C. B., Venitelli Z., Steinman H. M. 2007; Environmental mimics and the Lvh type IVA secretion system contribute to virulence-related phenotypes of Legionella pneumophila . Infect Immun 75:723–735
    [Google Scholar]
  3. Broich M., Rydzewski K., McNealy T. L., Marre R., Flieger A. 2006; The global regulatory proteins LetA and RpoS control phospholipase A, lysophospholipase A, acyltransferase, and other hydrolytic activities of Legionella pneumophila JR32. J Bacteriol 188:1218–1226
    [Google Scholar]
  4. Cianciotto N. P. 2005; Type II secretion: a protein secretion system for all seasons. Trends Microbiol 13:581–588
    [Google Scholar]
  5. De Buck E., Maes L., Meyen E., Van Mellaert L., Geukens N., Anné J., Lammertyn E. 2005; Legionella pneumophila Philadelphia-1 tatB and tatC affect intracellular replication and biofilm formation. Biochem Biophys Res Commun 331:1413–1420
    [Google Scholar]
  6. De Buck E., Vranckx L., Meyen E., Maes L., Vandersmissen L., Anné J., Lammertyn E. 2007; The twin-arginine translocation pathway is necessary for correct membrane insertion of the Rieske Fe/S protein in Legionella pneumophila . FEBS Lett 581:259–264
    [Google Scholar]
  7. DebRoy S., Dao J., Soderberg M., Rossier O., Cianciotto N. P. 2006; Legionella pneumophila type II secretome reveals unique exoproteins and a chitinase that promotes bacterial persistence in the lung. Proc Natl Acad Sci U S A 103:19146–19151
    [Google Scholar]
  8. Edelstein P. H., Edelstein M. A., Higa F., Falkow S. 1999; Discovery of virulence genes of Legionella pneumophila by using signature tagged mutagenesis in a guinea pig pneumonia model. Proc Natl Acad Sci U S A 96:8190–8195
    [Google Scholar]
  9. Gal-Mor O., Segal G. 2003; Identification of CpxR as a positive regulator of icm and dot virulence genes of Legionella pneumophila . J Bacteriol 185:4908–4919
    [Google Scholar]
  10. Gerlach R. G., Hensel M. 2007; Protein secretion systems and adhesins: the molecular armory of Gram-negative pathogens. Int J Med Microbiol 297:401–415
    [Google Scholar]
  11. Geukens N., De Buck E., Meyen E., Maes L., Vranckx L., Van Mellaert L., Anne J., Lammertyn E. 2006; The type II signal peptidase of Legionella pneumophila . Res Microbiol 157:836–841
    [Google Scholar]
  12. Jacobi S., Heuner K. 2003; Description of a putative type I secretion system in Legionella pneumophila . Int J Med Microbiol 293:349–358
    [Google Scholar]
  13. Lammertyn E., Anné J. 2004; Protein secretion in Legionella pneumophila and its relation to virulence. FEMS Microbiol Lett 238:273–279
    [Google Scholar]
  14. Lammertyn E., Van Mellaert L., Meyen E., Lebeau I., De Buck E., Anne J., Geukens N. 2004; Molecular and functional characterization of type I signal peptidase from Legionella pneumophila . Microbiology 150:1475–1483
    [Google Scholar]
  15. Lee P. A., Tullman-Ercek D., Georgiou G. 2006; The bacterial twin-arginine translocation pathway. Annu Rev Microbiol 60:373–395
    [Google Scholar]
  16. Liles M. R., Viswanathan V. K., Cianciotto N. P. 1998; Identification and temperature regulation of Legionella pneumophila genes involved in type IV pilus biogenesis and type II protein secretion. Infect Immun 66:1776–1782
    [Google Scholar]
  17. Lucas C. E., Brown E., Fields B. S. 2006; Type IV pili and type II secretion play a limited role in Legionella pneumophila biofilm colonization and retention. Microbiology 152:3569–3573
    [Google Scholar]
  18. Molmeret M., Santic M., Asare R., Carabeo R. A., Kwaik Y. A. 2007; Rapid escape of the dot / icm mutants of Legionella pneumophila into the cytosol of mammalian and protozoan cells. Infect Immun 75:3290–3304
    [Google Scholar]
  19. Ninio S., Roy C. R. 2007; Effector proteins translocated by Legionella pneumophila : strength in numbers. Trends Microbiol 15:372–380
    [Google Scholar]
  20. Ridenour D. A., Cirillo S. L., Feng S., Samrakandi M. M., Cirillo J. D. 2003; Identification of a gene that affects the efficiency of host cell infection by Legionella pneumophila in a temperature-dependent fashion. Infect Immun 71:6256–6263
    [Google Scholar]
  21. Rossier O., Cianciotto N. P. 2005; The Legionella pneumophila tatB gene facilitates secretion of phospholipase C, growth under iron-limiting conditions, and intracellular infection. Infect Immun 73:2020–2032
    [Google Scholar]
  22. Rossier O., Starkenburg S. R., Cianciotto N. P. 2004; Legionella pneumophila type II protein secretion promotes virulence in the A/J mouse model of Legionnaires' disease pneumonia. Infect Immun 72:310–321
    [Google Scholar]
  23. Samrakandi M. M., Cirillo S. L., Ridenour D. A., Bermudez L. E., Cirillo J. D. 2002; Genetic and phenotypic differences between Legionella pneumophila strains. J Clin Microbiol 40:1352–1362
    [Google Scholar]
  24. Segal G., Shuman H. A. 1998; How is the intracellular fate of the Legionella pneumophila phagosome determined?. Trends Microbiol 6:253–255
    [Google Scholar]
  25. Segal G., Shuman H. A. 1999; Legionella pneumophila utilizes the same genes to multiply within Acanthamoeba castellanii and human macrophages. Infect Immun 67:2117–2124
    [Google Scholar]
  26. Segal G., Russo J. J., Shuman H. A. 1999; Relationships between a new type IV secretion system and the icm/dot virulence system of Legionella pneumophila . Mol Microbiol 34:799–809
    [Google Scholar]
  27. Segal G., Feldman M., Zusman T. 2005; The Icm/Dot type-IV secretion systems of Legionella pneumophila and Coxiella burnetii . FEMS Microbiol Rev 29:65–81
    [Google Scholar]
  28. Steinert M., Heuner K., Buchrieser C., Albert-Weissenberger C., Glockner G. 2007; Legionella pathogenicity: genome structure, regulatory networks and the host cell response. Int J Med Microbiol 297:577–587
    [Google Scholar]
  29. van der Sluis E. O., Driessen A. J. M. 2006; Stepwise evolution of the Sec machinery in Proteobacteria. Trends Microbiol 14:105–108
    [Google Scholar]
  30. Vincent C. D., Buscher B. A., Friedman J. R., Williams L. A., Bardill P., Vogel J. P. 2006a; Identification of non- dot / icm suppressors of the Legionella pneumophila Δ dotL lethality phenotype. J Bacteriol 188:8231–8243
    [Google Scholar]
  31. Vincent C. D., Friedman J. R., Jeong K. C., Buford E. C., Miller J. L., Vogel J. P. 2006b; Identification of the core transmembrane complex of the Legionella Dot/Icm type IV secretion system. Mol Microbiol 62:1278–1291
    [Google Scholar]
  32. Zusman T., Aloni G., Halperin E., Kotzer H., Degtyar E., Feldman M., Segal G. 2007; The response regulator PmrA is a major regulator of the icm / dot type IV secretion system in Legionella pneumophila and Coxiella burnetii . Mol Microbiol 63:1508–1523
    [Google Scholar]
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