1887

Abstract

SmpA is a small outer-membrane lipoprotein that is a component of the essential YaeT outer-membrane protein assembly complex. In serovar Typhimurium ( Typhimurium), expression of the gene was shown to be directed by two promoters, and . The more distal promoter, , is dependent upon the extracytoplasmic stress response sigma factor . An null mutant was constructed in Typhimurium SL1344 and was shown to be more sensitive than its wild-type parent to growth at high temperature and in the presence of sodium cholate, SDS plus EDTA, and the hydrophobic antibiotic rifampicin. The lack of SmpA in Typhimurium elicits a -dependent stress response. These findings are indicative of altered outer-membrane integrity in the mutant, probably due to a defect in outer-membrane protein biogenesis. SmpA was not important for entry or survival within murine macrophages; however, the Typhimurium mutant was attenuated in mice by both the oral and parenteral routes of infection, and SmpA appeared to be most important for the growth of Typhimurium at systemic sites.

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2008-03-01
2020-03-29
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