1887

Abstract

The adaptation of to elevated levels of copper ions requires the copper-inducible operon encoding a copper chaperone and efflux ATPase. Here we identify CsoR (formerly YvgZ) as the copper-sensing repressor that regulates the operon. CsoR binds with high affinity to an operator site overlapping the promoter and its binding is specifically inhibited by copper salts. As previously described, the YhdQ (CueR) protein also binds to the regulatory region, but genetic experiments indicate that this protein is not responsible for the copper-dependent regulation of this operon.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.2007/011742-0
2007-12-01
2024-12-06
Loading full text...

Full text loading...

/deliver/fulltext/micro/153/12/4123.html?itemId=/content/journal/micro/10.1099/mic.0.2007/011742-0&mimeType=html&fmt=ahah

References

  1. Balatri E., Banci L., Bertini I., Cantini F., Ciofi-Baffoni S. 2003; Solution structure of Sco1: a thioredoxin-like protein involved in cytochrome c oxidase assembly. Structure 11:1431–1443
    [Google Scholar]
  2. Banci L., Bertini I., Conte R. D., Markey J., Ruiz-Duenas F. J. 2001; Copper trafficking: the solution structure of Bacillus subtilis CopZ. Biochemistry 40:15660–15668
    [Google Scholar]
  3. Banci L., Bertini I., Ciofi-Baffoni S., Gonnelli L., Su X. 2003; Structural basis for the function of the N-terminal domain of the ATPase CopA from Bacillus subtilis . J Biol Chem 278:50506–50513
    [Google Scholar]
  4. Banci L., Bertini I., Cavallaro G., Rosato A. 2007; The functions of Sco proteins from genome-based analysis. J Proteome Res 6:1568–1579
    [Google Scholar]
  5. Cutting S., Vander Horn P. B. 1990 Molecular Biological Methods for Bacillus Chichester: Wiley;
  6. Gaballa A., Helmann J. D. 2003; Bacillus subtilis CPx-type ATPases: characterization of Cd, Zn, Co, and Cu efflux systems. Biometals 16:497–505
    [Google Scholar]
  7. Gaballa A., Cao M., Helmann J. D. 2003; Two MerR homologues that affect copper induction of the Bacillus subtilis copZA operon. Microbiology 149:3413–3421
    [Google Scholar]
  8. Imlay J. A. 2002; How oxygen damages microbes: oxygen tolerance and obligate anaerobiosis. Adv Microb Physiol 46:111–153
    [Google Scholar]
  9. Liu T., Ramesh A., Ma Z., Ward S. K., Zhang L., George G. N., Talaat A. M., Sacchettini J. C., Giedroc D. P. 2007; CsoR is a novel Mycobacterium tuberculosis copper-sensing transcriptional regulator. Nat Chem Biol 3:60–68
    [Google Scholar]
  10. Mattatall N. R., Jazairi J., Hill B. C. 2000; Characterization of YpmQ, an accessory protein required for the expression of cytochrome c oxidase in Bacillus subtilis . J Biol Chem 275:28802–28809
    [Google Scholar]
  11. Miller J. H. 1972; Assay of β -galactosidase. In Experiments in Molecular Genetics pp 352–355 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  12. Moszer I., Glaser P., Danchin A. 1995; SubtiList: a relational database for the Bacillus subtilis genome. Microbiology 141:261–268
    [Google Scholar]
  13. Outten F. W., Outten C. E., Hale J., O'Halloran T. V. 2000; Transcriptional activation of an Escherichia coli copper efflux regulon by the chromosomal MerR homologue. CueR. J Biol Chem 275:31024–31029
    [Google Scholar]
  14. Radford D. S., Kihlken M. A., Borrelly G. P., Harwood C. R., Le Brun N. E., Cavet J. S. 2003; CopZ from Bacillus subtilis interacts in vivo with a copper exporting CPx-type ATPase CopA. FEMS Microbiol Lett 220:105–112
    [Google Scholar]
  15. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  16. Stoyanov J. V., Hobman J. L., Brown N. L. 2001; CueR (YbbI) of Escherichia coli is a MerR family regulator controlling expression of the copper exporter CopA. Mol Microbiol 39:502–511
    [Google Scholar]
  17. Touati D. 2000; Iron and oxidative stress in bacteria. Arch Biochem Biophys 373:1–6
    [Google Scholar]
  18. Vander Horn P. B., Zahler S. A. 1992; Cloning and nucleotide sequence of the leucyl-tRNA synthetase gene of Bacillus subtilis . J Bacteriol 174:3928–3935
    [Google Scholar]
  19. Wach A. 1996; PCR-synthesis of marker cassettes with long flanking homology regions for gene disruptions in Scerevisiae . Yeast 12:259–265
    [Google Scholar]
/content/journal/micro/10.1099/mic.0.2007/011742-0
Loading
/content/journal/micro/10.1099/mic.0.2007/011742-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error