1887

Abstract

Filamentous members of the are commonly observed in activated sludge samples originating from both municipal and industrial wastewater treatment plants (WWTP), where they occasionally can cause bulking. Several oligonucleotide 16S rRNA-targeted probes were designed to target filaments with a needle-like appearance similar to . The design of these probes was based on an isolate and a sequence obtained from a micromanipulated filament. The abundance of filamentous was determined in 126 industrial samples applying already published and the newly developed probes. Small populations were found in 62 % of the WWTP investigated. However, only relatively few WWTP (13 %) contained large populations of filamentous potentially responsible for bulking incidences. The identity of the most abundant filamentous with morphology could be detected by probes CFB719, SAP-309 and the newly designed probe HHY-654. A comprehensive study on the ecophysiology of probe-defined populations was conducted on Danish and Czech samples. The studies revealed that they were specialized bacteria involved in degradation of sugars, e.g. glucose and -acetylglucosamine, and may participate in the conversion of lipopolysaccharides and peptidoglycan liberated by decaying cells. Many surface-associated exo-enzymes were excreted, e.g. chitinase, glucuronidase, esterase and phosphatase, supporting conversion of polysaccharides and possibly other released cell components. The role of filamentous bacteria with a -like morphology in the activated sludge ecosystem is discussed.

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2008-03-01
2020-04-05
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References

  1. Amann R. I.. 1995; In situ identification of micro-organisms by whole cell hybridization with rRNA-targeted nucleic acid probes. In Molecular Microbial Ecological Manual pp1–15 Edited by Akkermans A. D. L., van Elsas J. D., de Bruijn F. J.. London: Kluwer Academic Publications;
    [Google Scholar]
  2. Andreasen K., Nielsen P. H.. 1997; Application of microautoradiography to the study of substrate uptake by filamentous microorganisms in activated sludge. Appl Environ Microbiol63:3662–3668
    [Google Scholar]
  3. Andreasen K., Nielsen P. H.. 2000; Growth of Microthrix parvicella in nutrient removal activated sludge plants: studies of in situ physiology. Water Res34:1559–1569
    [Google Scholar]
  4. Barker D. J., Stuckey D. C.. 1999; A review of soluble microbial products (SMP) in wastewater treatment systems. Water Res33:3063–3082
    [Google Scholar]
  5. Baty A. M., Eastburn C. C., Diwu Z., Techkarnjanaruk S., Goodman A. E., Geesey G. G.. 2000; Differentiation of chitinase-active and non-chitinase-active subpopulations of a marine bacterium during chitin degradation. Appl Environ Microbiol66:3566–3573
    [Google Scholar]
  6. Baty A. M., Diwu Z., Dunham G., Eastburn C. C., Geesey G. G., Goodman A. E., Suci P. A., Techkarnjanaruk S.. 2001; Characterization of extracellular chitinolytic activity in biofilms. Methods Enzymol336:279–301
    [Google Scholar]
  7. Chiesa S. C., Irvine R. L.. 1985; Growth and control of filamentous microbes in activated sludge – an integrated hypothesis. Water Res19:471–479
    [Google Scholar]
  8. Eikelboom D. H.. 1975; Filamentous organisms observed in activated sludge. Water Res9:365–388
    [Google Scholar]
  9. Eikelboom D. H.. 2002; Process Control of Activated Sludge Plants by Microscopic Investigation London, UK: IWA Publishing;
    [Google Scholar]
  10. Eikelboom D. H.. 2006; Identification and Control of Filamentous Microorganisms in Industrial Wastewater Treatment Plants London, UK: IWA Publishing;
    [Google Scholar]
  11. Eikelboom D., Geurkink B.. 2000; Catalogue of Filamentous Microorganisms Occurring in Industrial Activated Sludge Plants TNO-MEP. Report R; 2001
    [Google Scholar]
  12. Eikelboom D. H., Geurkink B.. 2002; Filamentous micro-organisms observed in industrial activated sludge plants. Water Sci Technol46:535–542
    [Google Scholar]
  13. Eilers H., Pernthaler J., Peplies J., Glöckner F. O., Gerdts G., Amann R.. 2001; Isolation of novel pelagic bacteria from the German Bight and their seasonal contributions to surface picoplankton. Appl Environ Microbiol67:5134–5142
    [Google Scholar]
  14. Hugenholtz P., Huber T.. 2003; Chimeric 16S rDNA sequences of diverse origin are accumulating in the public databases. Int J Syst Evol Microbiol53:289–293
    [Google Scholar]
  15. Jenkins D., Richard M. G., Daigger G. T.. 2004; Manual on the Causes and Control of Activated Sludge Bulking, Foaming, and Other Solids Separation Problems London: Lewis Publishers;
    [Google Scholar]
  16. Kämpfer P.. 1995; Physiological and chemotaxonomic characterization of filamentous bacteria belonging to the genus Haliscomenobacter . Syst Appl Microbiol18:363–367
    [Google Scholar]
  17. Kindaichi T., Ito T., Okabe S.. 2004; Ecophysiological interaction between nitrifying bacteria and heterotrophic bacteria in autotrophic nitrifying biofilms as determined by microautoradiography-fluorescence in situ hybridization. Appl Environ Microbiol70:1641–1650
    [Google Scholar]
  18. Kragelund C., Nielsen J. L., Thomsen T. R., Nielsen P. H.. 2005; Ecophysiology of the filamentous Alphaproteobacterium Meganema perideroedes in activated sludge. FEMS Microbiol Ecol54:111–122
    [Google Scholar]
  19. Kragelund C., Kong Y., van der Waarde J., Thelen K., Eikelboom D., Tandoi V., Thomsen T. R., Nielsen P. H.. 2006; Ecophysiology of different filamentous Alphaproteobacteria species from industrial waste water treatment plants. Microbiology152:3003–3012
    [Google Scholar]
  20. Kragelund C., Levantesi C., Borger A., Thelen K., Eikelboom D., Tandoi V., Kong Y., van der Waarde J., Krooneman J.. other authors 2007; Identity, abundance and ecophysiology of different filamentous Chloroflexi species from activated sludge treatment plants. FEMS Microbiol Ecol59:671–682
    [Google Scholar]
  21. Krul J. M.. 1977; Experiments with Haliscomenobacter hydrossis in continuous culture without and with Zoogloea ramigera . Water Res11:197–204
    [Google Scholar]
  22. Lee N., Nielsen P. H., Andreasen K. H., Juretschko S., Nielsen J. L., Schleifer K. H., Wagner M.. 1999; Combination of fluorescent in situ hybridization and microautoradiography – a new tool for structure-function analyses in microbial ecology. Appl Environ Microbiol65:1289–1297
    [Google Scholar]
  23. Levantesi C., Beimfohr C., Geurkink B., Rossetti S., Thelen K., Krooneman J., Snaidr J., van der Waarde J., Tandoi V.. 2004; Filamentous Alphaproteobacteria associated with bulking in industrial wastewater treatment plants. Syst Appl Microbiol27:716–727
    [Google Scholar]
  24. Levantesi C., Rossetti S., Beimfohr C., Thelen K., Krooneman J., van der Waarde J., Tandoi V.. 2006; Description of filamentous bacteria present in industrial activated sludge WWTPs by conventional and molecular methods. Water Sci Technol54:129–137
    [Google Scholar]
  25. Loy A., Horn M., Wagner M.. 2003; probeBase: an online resource for rRNA-targeted oligonucleotide probes. Nucleic Acids Res31:514–516
    [Google Scholar]
  26. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S.. other authors 2004; arb: a software environment for sequence data. Nucleic Acids Res32:1363–1371
    [Google Scholar]
  27. Lydell C., Dowell L., Sikaroodi M., Gillevet P., Emerson D.. 2004; A population survey of members of the phylum Bacteroidetes isolated from salt marsh sediments along the East Coast of the United States. Microb Ecol48:263–273
    [Google Scholar]
  28. Manz W., Amann R., Ludwig W., Vancanneyt M., Schleifer K. H.. 1996; Application of a suite of 16S rRNA-specific oligonucleotide probes designed to investigate bacteria of the phylum Cytophaga-Flavobacter-Bacteroides in the natural environment. Microbiology142:1097–1106
    [Google Scholar]
  29. Mulder E. G., Deinema M. H.. 1992; The sheathed bacteria. In The Prokaryotes: a Handbook on the Biology of Bacteria: Ecophysiology, Isolation, Identification, Application , 2nd edn. pp2612–2624 Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H.. New York: Springer-Verlag;
    [Google Scholar]
  30. Nielsen P. H., de Muro M. A., Nielsen J. L.. 2000; Studies on the in situ physiology of Thiothrix spp. present in activated sludge. Environ Microbiol2:389–398
    [Google Scholar]
  31. Ouverney C. C., Fuhrman J. A.. 1999; Combined microautoradiography-16S rRNA probe technique for determination of radioisotope uptake by specific microbial cell types in situ. Appl Environ Microbiol65:1746–1752
    [Google Scholar]
  32. Reichenbach H.. 1999; The Order Cytophagales. In The Prokaryotes: an Evolving Electronic Resource for the Microbiological Community , 3rd edn. Edited by Dworkin M. New York: Springer-Verlag;
    [Google Scholar]
  33. Schauer M., Hahn M. W.. 2005; Diversity and phylogenetic affiliations of morphologically conspicuous large filamentous bacteria occurring in the pelagic zones of a broad spectrum of freshwater habitats. Appl Environ Microbiol71:1931–1940
    [Google Scholar]
  34. Snaidr J., Fuchs B., Wallner G., Wagner M., Schleifer K. H., Amann R.. 1999; Phylogeny and in situ identification of a morphologically conspicuous bacterium, Candidatus Magnospira bakii, present at very low frequency in activated sludge. Environ Microbiol1:125–135
    [Google Scholar]
  35. van der Waarde J., Krooneman J., Geurkink B., van der Werf A., Eikelboom D., Beimfohr C., Snaidr J., Levantesi C., Tandoi V.. 2002; Molecular monitoring of bulking sludge in industrial wastewater treatment plants. Water Sci Technol46:551–558
    [Google Scholar]
  36. van Veen W. L.. 1973; Bacteriology of activated-sludge, in particular filamentous bacteria. Antonie Van Leeuwenhoek39:189–205
    [Google Scholar]
  37. van Veen W. L., van der Kooij D., Geuze E. C., van der Vlies A. W.. 1973; Investigations on sheathed bacterium Haliscomenobacter hydrossis gen. nov., sp. nov., isolated from activated sludge. Antonie Van Leeuwenhoek39:207–216
    [Google Scholar]
  38. van Veen W. L., Krul J. M., Bulder C. J. E. A.. 1982; Some growth-parameters of Haliscomenobacter hydrossis (Syn. Streptothrix hyalina ) a bacterium occurring in bulking activated sludge. Water Res16:531–534
    [Google Scholar]
  39. Wagner M., Amann R., Kämpfer P., Assmus B., Hartmann A., Hutzler P., Springer N., Schleifer K. H.. 1994; Identification and in situ detection of Gram-negative filamentous bacteria in activated sludge. Syst Appl Microbiol17:405–417
    [Google Scholar]
  40. Wanner J.. 1994; Activated Sludge Bulking and Foaming Control Basel: Technomic Publishing Company;
    [Google Scholar]
  41. Weller R., Glöckner F. O., Amann R.. 2000; 16S rRNA-targeted oligonucleotide probes for the in situ detection of members of the phylum Cytophaga-Flavobacterium-Bacteroides . Syst Appl Microbiol23:107–114
    [Google Scholar]
  42. Williams T. M., Unz R. F.. 1985; Isolation and characterization of filamentous bacteria present in bulking activated-sludge. Appl Microbiol Biotechnol22:273–282
    [Google Scholar]
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