%0 Journal Article %A Jakimowicz, Dagmara %A Brzostek, Anna %A Rumijowska-Galewicz, Anna %A Żydek, Paulina %A Dołzbłasz, Alicja %A Smulczyk-Krawczyszyn, Aleksandra %A Zimniak, Tomasz %A Wojtasz, Łukasz %A Zawilak-Pawlik, Anna %A Kois, Agnieszka %A Dziadek, Jarosław %A Zakrzewska-Czerwińska, Jolanta %T Characterization of the mycobacterial chromosome segregation protein ParB and identification of its target in Mycobacterium smegmatis %D 2007 %J Microbiology, %V 153 %N 12 %P 4050-4060 %@ 1465-2080 %R https://doi.org/10.1099/mic.0.2007/011619-0 %K EMSA, electrophoretic mobility shift assay %K GST, glutathione S-transferase %K Mt, M. tuberculosis %K Ms, M. smegmatis %I Microbiology Society, %X Bacterial chromosomes (though not Escherichia coli and some other γ-proteobacterial chromosomes) contain parS sequences and parAB genes encoding partitioning proteins, i.e. ParA (ATPase) and ParB (DNA-binding proteins) that are components of the segregation machinery. Here, mycobacterial parABS elements were characterized for the first time. parAB genes are not essential in Mycobacterium smegmatis; however, elimination or overexpression of ParB protein causes growth inhibition. Deletion of parB also leads to a rather severe chromosome segregation defect: up to 10 % of the cells were anucleate. Mycobacterial ParB protein uses three oriC-proximal parS sequences as targets to organize the origin region into a compact nucleoprotein complex. Formation of such a complex involves ParB–ParB interactions and is assisted by ParA protein. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.2007/011619-0