1887

Abstract

Bacterial chromosomes (though not and some other -proteobacterial chromosomes) contain sequences and genes encoding partitioning proteins, i.e. ParA (ATPase) and ParB (DNA-binding proteins) that are components of the segregation machinery. Here, mycobacterial elements were characterized for the first time. genes are not essential in however, elimination or overexpression of ParB protein causes growth inhibition. Deletion of also leads to a rather severe chromosome segregation defect: up to 10 % of the cells were anucleate. Mycobacterial ParB protein uses three -proximal sequences as targets to organize the origin region into a compact nucleoprotein complex. Formation of such a complex involves ParB–ParB interactions and is assisted by ParA protein.

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2007-12-01
2020-08-15
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