1887

Abstract

produces the mycotoxin fumonisin B (FB) on maize kernels. In this study, we identified a putative protein phosphatase gene in , and investigated its role in FB regulation. Previous work has shown that expression is elevated in an FB-suppressing genetic background. Thus, we hypothesized that is negatively associated with FB production. To test this hypothesis, we generated a knockout mutant, PP179, and studied the effects of gene deletion on FB biosynthesis and fungal development. PP179 showed elevated expression of genes, and in turn produced higher levels of FB than the wild-type progenitor. Other significant mutant phenotypes included reduced radial growth on agar plates, reduced conidia germination rates, significantly increased macroconidia formation, and hyphal swelling. To verify that these phenotypes were directly due to deletion, we complemented PP179 with the wild-type gene. The complemented strain PPC4 showed expression and FB production similar to that of the wild-type, providing evidence that is negatively associated with FB biosynthesis. Other PP179 phenotypes, such as macroconidiation and hyphal swelling, were also restored to that of wild-type progenitor. Furthermore, we complemented PP179 strain with wild-type gene, demonstrating that Cpp1 and PPE-1 proteins are functionally conserved. Pleiotropic effects of deletion led us to hypothesize that is associated with multiple downstream signalling pathways in . Identification and functional characterization of downstream Cpp1-interacting proteins are necessary to better understand the complex regulatory mechanisms associated with Cpp1.

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2008-01-01
2019-11-13
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vol. , part 1, pp. 326-336

Descriptions of PP179 complementation using the YEC3 construct (section 1), of how exogenous application of protein phosphatase inhibitor okadaic acid did not induce hyphal swelling in (section 2) and of the YEC3 construct (Fig. S1) are available here.



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