1887

Abstract

, the causative agent of swine enzootic pneumonia, colonizes the cilia of swine lungs, causing ciliostasis and cell death. is a component of the porcine respiratory disease complex (PRDC) and is especially problematic for the finishing swine industry, causing the loss of hundreds of millions of dollars in farm revenues worldwide. For successful infection, must effectively resist oxidative stresses due to the release of oxidative compounds from neutrophils and macrophages during the host's immune response. However, the mechanism that uses to avert the host response is still unclear. To gain a better understanding of the transcriptional responses of under oxidative stress, cultures were grown to early exponential phase and exposed to 0.5 % hydrogen peroxide for 15 min. RNA samples from these cultures were collected and compared to RNA samples from control cultures using two-colour PCR-based microarrays. This study revealed significant downregulation of important glycolytic pathway genes and gene transcription proteins, as well as a protein known to activate oxidative stressor cascades in neutrophils. Sixty-nine per cent of the upregulated genes were hypothetical with no known function. This study has also revealed significantly differentially expressed genes common to other environmental stress responses, indicating that further investigation of universal stress response genes of is merited.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.2007/011387-0
2007-11-01
2019-10-19
Loading full text...

Full text loading...

/deliver/fulltext/micro/153/11/3785.html?itemId=/content/journal/micro/10.1099/mic.0.2007/011387-0&mimeType=html&fmt=ahah

References

  1. Amass, S. F., Clark, L. K., van Alstine, W. G., Bowersock, T. L., Murphy, D. A., Knox, K. E. & Albregts, S. R. ( 1994; ). Interaction of Mycoplasma hyopneumoniae and Pasteurella multocida infections in swine. J Am Vet Med Assoc 204, 102–107.
    [Google Scholar]
  2. Baskerville, A. ( 1972; ). Development of the early lesions in experimental enzootic pneumonia of pig: an ultrastructural and histological study. Res Vet Sci 13, 570–578.
    [Google Scholar]
  3. Boyce, J. D., Wilkie, I., Harper, M., Paustian, M. L., Kapur, V. & Adler, B. ( 2004; ). Genomic-scale analysis of Pasteurella multocida gene expression during growth within liver tissue of chickens with fowl cholera. Microbes Infect 6, 290–298.[CrossRef]
    [Google Scholar]
  4. DeBey, M. C. & Ross, R. F. ( 1994; ). Ciliostasis and loss of cilia induced by Mycoplasma hyopneumoniae in porcine tracheal organ cultures. Infect Immun 62, 5312–5318.
    [Google Scholar]
  5. Dudley, A. M., Aach, J., Steffen, M. A. & Church, G. M. ( 2002; ). Measuring absolute expression with microarrays with a calibrated reference sample and an extended signal intensity range. Proc Natl Acad Sci U S A 99, 7554–7559.[CrossRef]
    [Google Scholar]
  6. Farr, S. B. & Kogoma, T. ( 1991; ). Oxidative stress responses in Escherichia coli and Salmonella typhimurium. Microbiol Rev 55, 561–585.
    [Google Scholar]
  7. Gallup, J. M. & Ackermann, M. R. ( 2006; ). Addressing fluorogenic real-time qPCR inhibition using the novel custom Excel file system ‘Focusfield2-6GallupqPCRSet-upTool-001’ to attain consistently high fidelity qPCR reactions. Biol Proced Online 8, 87–152.[CrossRef]
    [Google Scholar]
  8. Gusarov, I. & Nudler, E. ( 2005; ). NO-mediated cytoprotection: instant adaptation to oxidative stress in bacteria. Proc Natl Acad Sci U S A 102, 13855–13860.[CrossRef]
    [Google Scholar]
  9. Madsen, M. L., Nettleton, D., Thacker, E. L., Edwards, R. & Minion, F. C. ( 2006a; ). Transcriptional profiling of Mycoplasma hyopneumoniae during heat shock using microarrays. Infect Immun 74, 160–166.[CrossRef]
    [Google Scholar]
  10. Madsen, M. L., Nettleton, D., Thacker, E. L. & Minion, F. C. ( 2006b; ). Transcriptional profiling of Mycoplasma hyopneumoniae during iron depletion using microarrays. Microbiology 152, 937–944.[CrossRef]
    [Google Scholar]
  11. Messier, S., Ross, R. F. & Paul, P. S. ( 1990; ). Humoral and cellular immune responses of pigs inoculated with Mycoplasma hyopneumoniae. Am J Vet Res 51, 52–58.
    [Google Scholar]
  12. Mey, A. R., Wyckoff, E. E., Kanukurthy, V., Fisher, C. R. & Payne, S. M. ( 2005; ). Iron and Fur regulation in Vibrio cholerae and the role of Fur in virulence. Infect Immun 73, 8167–8178.[CrossRef]
    [Google Scholar]
  13. Mosig, M. O., Lipkin, E., Galina, K., Tchourzyna, E., Soller, M. & Friedmann, A. ( 2001; ). A whole genome scan for quantitative trait loci affecting milk protein percentage in Israeli-Holstein cattle, by means of selective milk DNA pooling in a daughter design, using an adjusted false discovery rate criterion. Genetics 157, 1683–1698.
    [Google Scholar]
  14. Raghavachari, N., Bao, Y. P., Li, G., Xie, X. & Mèuller, U. R. ( 2003; ). Reduction of autofluorescence on DNA microarrays and slide surfaces by treatment with sodium borohydride. Anal Biochem 312, 101–105.[CrossRef]
    [Google Scholar]
  15. Sarradell, J., Andrada, M., Ramâirez, A. S., Fernâandez, A., Gâomez-Villamandos, J. C., Jover, A., Lorenzo, H., Herrâaez, P. & Rodrâiguez, F. ( 2003; ). A morphologic and immunohistochemical study of the bronchus-associated lymphoid tissue of pigs naturally infected with Mycoplasma hyopneumoniae. Vet Pathol 40, 395–404.[CrossRef]
    [Google Scholar]
  16. Storey, J. D. & Tibshirani, R. ( 2003; ). Statistical significance for genomewide studies. Proc Natl Acad Sci U S A 100, 9440–9445.[CrossRef]
    [Google Scholar]
  17. Storz, G. & Toledano, M. B. ( 1994; ). Regulation of bacterial gene expression in response to oxidative stress. In Bacterial Pathogenesis, Part B, Interaction of Pathogenic Bacteria with Host Cells, pp. 196–207. Edited by V. L. Clark & P. M. Bavoil. New York: Academic Press.
  18. Thacker, E. L., Halbur, P. G., Ross, R. F., Thanawongnuwech, R. & Thacker, B. J. ( 1999; ). Mycoplasma hyopneumoniae potentiation of porcine reproductive and respiratory syndrome virus-induced pneumonia. J Clin Microbiol 37, 620–627.
    [Google Scholar]
  19. Wang, G., Hong, Y., Olczak, A., Maier, S. E. & Maier, R. J. ( 2006; ). Dual roles of Helicobacter pylori NapA in inducing and combating oxidative stress. Infect Immun 74, 6839–6846.[CrossRef]
    [Google Scholar]
  20. Wolfinger, R. D., Gibson, G., Wolfinger, E. D., Bennett, L., Hamadeh, H., Bushel, P., Afshari, C. & Paules, R. S. ( 2001; ). Assessing gene significance from cDNA microarray expression data via mixed models. J Comput Biol 8, 625–637.[CrossRef]
    [Google Scholar]
  21. Zheng, M., Wang, X., Templeton, L. J., Smulski, D. R., LaRossa, R. A. & Storz, G. ( 2001; ). DNA microarray-mediated transcriptional profiling of the Escherichia coli response to hydrogen peroxide. J Bacteriol 183, 4562–4570.[CrossRef]
    [Google Scholar]
  22. Zielinski, G. C. & Ross, R. F. ( 1993; ). Adherence of Mycoplasma hyopneumoniae to porcine ciliated respiratory tract cells. Am J Vet Res 54, 1262–1269.
    [Google Scholar]
  23. Zou, N. & Dybvig, K. ( 2002; ). DNA replication, repair and stress response. In Molecular Biology and Pathogenicity of Mycoplasmas, pp. 303–321. Edited by S. Razin & R. Herrmann. New York: Kluwer Academic/Plenum.
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.2007/011387-0
Loading
/content/journal/micro/10.1099/mic.0.2007/011387-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error