1887

Abstract

Variable surface protein Vmm and five Vmm-type proteins from subsp. SC were analysed to determine whether these proteins are expressed in animals affected by contagious bovine pleuropneumonia (CBPP) and . Recombinant versions of these proteins were constructed and expressed in after mutation of the TGA Trp codons to TGG. These proteins were then analysed by dot and Western blotting with sera from CBPP-affected cattle. Furthermore, affinity-purified polyclonal antibodies to the recombinant proteins were used in Western and colony blotting to look for expression of the putative Vmm-type proteins in cultured SC. This study demonstrates that immunoglobulins in CBPP sera recognize all putative Vmm-type proteins tested, indicating that these proteins or their homologues are expressed by mycoplasmas during natural infections. Vmm and one of the putative Vmm-type proteins showed variable expression .

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2008-02-01
2019-12-06
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Primers used in PCR, mutagenesis and DNA sequencing [PDF file](63 KB)



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