1887

Abstract

The chemolithoautotroph has two genes predicted to encode outer-membrane (OM) ferrioxamine transporters. Expression of the ferrioxamine uptake system required induction, as shown by the shorter lag phase in ferrioxamine-containing cultures when ferrioxamine-exposed cells were used as an inoculum. The two OM ferrioxamine siderophore transporters encoded by (NE1097) and (NE1088) were produced only in cells grown in Fe-limited ferrioxamine-containing medium. The inactivation of , singly or in combination with , prevented growth in Fe-limited medium containing excess desferrioxamine (DFX). The -disrupted single mutant grew poorly in the regular Fe-limited (0.2 μM) medium with 10 μM DFX, but grew well when the Fe level was raised to 1.0 μM with 10 μM DFX. For efficient acquisition of Fe-loaded ferrioxamine, needs both ferrioxamine transporters FoxA and FoxA. FoxA probably regulates its own production, and it controls the production of FoxA as well.

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2007-12-01
2021-07-31
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