1887

Abstract

The gene, which encodes the -aminobutyric acid (GABA) and -aminolaevulinic acid (ALA) permease, is well known to be regulated by the nitrogen source. Its expression levels are low in the presence of a rich nitrogen source but are higher when a poor nitrogen source is used. In addition, GABA can induce expression when cells are grown with proline but not when they are grown with ammonium. Although vast amounts of evidence have been gathered about regulation by nitrogen, little is known about its regulation by the carbon source. Using glucose and acetate as rich and poor carbon source respectively, this work aimed to shed light on hitherto unclear aspects of the regulation of this gene. In poor nitrogen conditions, cells grown with acetate were found to have higher basal expression levels than those grown with glucose, and did not show induction in response to GABA. Analysis of the expression and subcellular localization of the transcription factors that regulate as well as partial deletions and site-directed mutations of the promoter region suggested that there are two parallel pathways that act in regulating this gene by the carbon source. Furthermore, the results demonstrate the existence of a new factor operating in regulation.

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2007-11-01
2019-11-21
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