1887

Abstract

is a marine yeast that has to cope with different stress situations. Since changes in membrane properties can play an important function in adaptation, we have examined the fluidity and lipid composition of purified plasma membranes of grown at different external pH values and salt concentrations. Growth at low pH caused an increase in the sterol-to-phospholipid ratio and a decrease in fatty acid unsaturation which was reflected in decreased fluidity of the plasma membrane. High levels of NaCl increased the sterol-to-phospholipid ratio and fatty acid unsaturation, but did not significantly affect fluidity. The sterol-to-phospholipid ratios obtained in grown under any of these conditions were similar to the ratios that have been reported for halophilic/halotolerant black yeasts, but much smaller than those observed in the model yeast .

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.2007/009563-0
2007-10-01
2020-11-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/153/10/3586.html?itemId=/content/journal/micro/10.1099/mic.0.2007/009563-0&mimeType=html&fmt=ahah

References

  1. Almagro A., Prista C., Castro S., Quintas C., Madeira-Lopes A., Ramos J., Loureiro-Dias M. C.. 2000; Effects of salts on Debaryomyces hansenii and Saccharomyces cerevisiae under stress conditions. Int J Food Microbiol56:191–197
    [Google Scholar]
  2. Andreishcheva E. N., Isakova E. P., Sidorov N. N., Abramova N. B., Ushakova N. A., Shaposhnikov G. L., Soares M. I. M., Zvyagilskaya R. A.. 1999; Adaptation to salt stress in a salt-tolerant strain of the yeast Yarrowia lipolytica . Biochemistry (Mosc)64:1061–1067
    [Google Scholar]
  3. Drici-Cachon Z., Cavin J. F., Divies C.. 1996; Effect of pH and age of culture on cellular fatty acid composition of Leuconostoc oenos . Lett App Microbiol22:331–334
    [Google Scholar]
  4. Fozo E. M., Kajfasz J. K., Quivey R. G.. 2004; Low pH-induced membrane fatty acid alterations in oral bacteria. FEMS Microbiol Lett238:291–295
    [Google Scholar]
  5. Hosono K.. 1992; Effect of salt stress on lipid composition and membrane fluidity of the salt-tolerant yeast Zygosaccharomyces rouxii . J Gen Microbiol138:91–96
    [Google Scholar]
  6. Kates M.. 1986; Lipid extraction procedures. In Techniques of Lipidology: Isolation, Analysis and Identification of Lipids pp100–115 Amsterdam: Elsevier;
  7. Khaware R. K., Koul A., Prasad R.. 1995; High membrane fluidity is related to NaCl stress in Candida membranefaciens . Biochem Mol Biol Int35:875–880
    [Google Scholar]
  8. Norkrans B.. 1966; Studies on marine occuring yeast: growth related to pH, NaCl concentration and temperature. Arch Mikrobiol54:374–392
    [Google Scholar]
  9. Panaretou B., Piper P.. 1996; Isolation of yeast plasma membranes. In Yeast Protocols vol. 53 pp117–121 Edited by Evans I. H. Totowa, NJ: Humana Press;
  10. Prista C., Almagro A., Loureiro-Dias M. C., Ramos J.. 1997; Physiological basis for the high salt tolerance of Debaryomyces hansenii . Appl Environ Microbiol63:4005–4009
    [Google Scholar]
  11. Prista C., Loureiro-Dias M. C., Montiel V., Garcia R., Ramos J.. 2005; Mechanisms underlying the halotolerant way of Debaryomyces hansenii . FEMS Yeast Res5:693–701
    [Google Scholar]
  12. Russell N. J.. 1989a; Adaptive modifications in membranes of halotolerant and halophilic microorganisms. J Bioenerg Biomembr21:93–113
    [Google Scholar]
  13. Russell N. J.. 1989b; Structural and Functional Role of Lipids vol. 2 New York: Academic Press;
  14. Russell N. J., Evans R. I., ter Steeg P. F., Hellemons J., Verheul A., Abee T.. 1995; Membranes as a target for stress adaptation. Int J Food Microbiol28:255–261
    [Google Scholar]
  15. Serrano R.. 1988; H+-ATPase from plasma membranes of Saccharomyces cerevisiae and Avena sativa roots. Purification and Reconstitution. Biomembranes Part Q, ATP-Driven Pumps and Related Transport Calcium, Proton and Potassium Pumps vol. 157: San Diego: Academic Press;
    [Google Scholar]
  16. Sharma S. C., Raj D., Forouzandeh M., Bansal M. P.. 1996; Salt-induced changes in lipid composition and ethanol tolerance in Saccharomyces cerevisiae . Appl Biochem Biotechnol56:189–195
    [Google Scholar]
  17. Strancar J., Sentjurc M., Schara M.. 2000; Fast and accurate characterization of biological membranes by EPR spectral simulations of nitroxides. J Magn Reson142:254–265
    [Google Scholar]
  18. Thomé P. E.. 2004; Isolation of a GPD gene from Debaryomyces hansenii encoding a glycerol 3-phosphate dehydrogenase (NAD+. Yeast21:119–126
    [Google Scholar]
  19. Thomé-Ortiz P. E., Peña A., Ramírez J.. 1998; Monovalent cation fluxes and physiological changes of Debaryomyces hansenii grown at high concentrations of KCl and NaCl. Yeast14:1355–1371
    [Google Scholar]
  20. Tunblad-Johansson I., Adler L.. 1987; Effect of sodium chloride concentration on phospholipid fatty acid composition of yeasts differing in osmotolerance. FEMS Microbiol Lett43:275–278
    [Google Scholar]
  21. Tunblad-Johansson I., Andre L., Adler L.. 1987; The sterol and phospholipid composition of the salt-tolerant yeast Debaryomyces hansenii grown at various concentrations of NaCl. Biochim Biophys Acta921:116–123
    [Google Scholar]
  22. Turk M., Mejanelle L., Sentjurc M., Grimalt J. O., Gunde-Cimerman N., Plemenitaš A.. 2004; Salt-induced changes in lipid composition and membrane fluidity of halophilic yeast-like melanized fungi. Extremophiles8:53–61
    [Google Scholar]
  23. Turk M., Abramović Z., Plemenitaš A., Gunde-Cimerman N.. 2007; Salt stress and plasma-membrane fluidity in selected extremophilic yeasts and yeast-like fungi. FEMS Yeast Res7:550–557
    [Google Scholar]
  24. Yoshikawa S., Mitsui N., Chikara K. I., Hashimoto H., Shimosaka M., Okazaki M.. 1995; Effect of salt stress on plasma membrane permeability and lipid saturation in the salt-tolerant yeast Zygosaccharomyces rouxii . J Ferment Bioeng80:131–135
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.2007/009563-0
Loading
/content/journal/micro/10.1099/mic.0.2007/009563-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error