1887

Abstract

is a marine yeast that has to cope with different stress situations. Since changes in membrane properties can play an important function in adaptation, we have examined the fluidity and lipid composition of purified plasma membranes of grown at different external pH values and salt concentrations. Growth at low pH caused an increase in the sterol-to-phospholipid ratio and a decrease in fatty acid unsaturation which was reflected in decreased fluidity of the plasma membrane. High levels of NaCl increased the sterol-to-phospholipid ratio and fatty acid unsaturation, but did not significantly affect fluidity. The sterol-to-phospholipid ratios obtained in grown under any of these conditions were similar to the ratios that have been reported for halophilic/halotolerant black yeasts, but much smaller than those observed in the model yeast .

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2007-10-01
2019-11-22
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References

  1. Almagro, A., Prista, C., Castro, S., Quintas, C., Madeira-Lopes, A., Ramos, J. & Loureiro-Dias, M. C. ( 2000; ). Effects of salts on Debaryomyces hansenii and Saccharomyces cerevisiae under stress conditions. Int J Food Microbiol 56, 191–197.[CrossRef]
    [Google Scholar]
  2. Andreishcheva, E. N., Isakova, E. P., Sidorov, N. N., Abramova, N. B., Ushakova, N. A., Shaposhnikov, G. L., Soares, M. I. M. & Zvyagilskaya, R. A. ( 1999; ). Adaptation to salt stress in a salt-tolerant strain of the yeast Yarrowia lipolytica. Biochemistry (Mosc) 64, 1061–1067.
    [Google Scholar]
  3. Drici-Cachon, Z., Cavin, J. F. & Divies, C. ( 1996; ). Effect of pH and age of culture on cellular fatty acid composition of Leuconostoc oenos. Lett App Microbiol 22, 331–334.[CrossRef]
    [Google Scholar]
  4. Fozo, E. M., Kajfasz, J. K. & Quivey, R. G. ( 2004; ). Low pH-induced membrane fatty acid alterations in oral bacteria. FEMS Microbiol Lett 238, 291–295.[CrossRef]
    [Google Scholar]
  5. Hosono, K. ( 1992; ). Effect of salt stress on lipid composition and membrane fluidity of the salt-tolerant yeast Zygosaccharomyces rouxii. J Gen Microbiol 138, 91–96.[CrossRef]
    [Google Scholar]
  6. Kates, M. ( 1986; ). Lipid extraction procedures. In Techniques of Lipidology: Isolation, Analysis and Identification of Lipids, pp. 100–115. Amsterdam: Elsevier.
  7. Khaware, R. K., Koul, A. & Prasad, R. ( 1995; ). High membrane fluidity is related to NaCl stress in Candida membranefaciens. Biochem Mol Biol Int 35, 875–880.
    [Google Scholar]
  8. Norkrans, B. ( 1966; ). Studies on marine occuring yeast: growth related to pH, NaCl concentration and temperature. Arch Mikrobiol 54, 374–392.[CrossRef]
    [Google Scholar]
  9. Panaretou, B. & Piper, P. ( 1996; ). Isolation of yeast plasma membranes, In Yeast Protocols vol. 53, pp. 117–121. Edited by I. H. Evans. Totowa, NJ: Humana Press.
  10. Prista, C., Almagro, A., Loureiro-Dias, M. C. & Ramos, J. ( 1997; ). Physiological basis for the high salt tolerance of Debaryomyces hansenii. Appl Environ Microbiol 63, 4005–4009.
    [Google Scholar]
  11. Prista, C., Loureiro-Dias, M. C., Montiel, V., Garcia, R. & Ramos, J. ( 2005; ). Mechanisms underlying the halotolerant way of Debaryomyces hansenii. FEMS Yeast Res 5, 693–701.[CrossRef]
    [Google Scholar]
  12. Russell, N. J. ( 1989a; ). Adaptive modifications in membranes of halotolerant and halophilic microorganisms. J Bioenerg Biomembr 21, 93–113.[CrossRef]
    [Google Scholar]
  13. Russell, N. J. ( 1989b; ). Structural and Functional Role of Lipids, vol. 2. New York: Academic Press.
  14. Russell, N. J., Evans, R. I., ter Steeg, P. F., Hellemons, J., Verheul, A. & Abee, T. ( 1995; ). Membranes as a target for stress adaptation. Int J Food Microbiol 28, 255–261.[CrossRef]
    [Google Scholar]
  15. Serrano, R. ( 1988; ). H+-ATPase from plasma membranes of Saccharomyces cerevisiae and Avena sativa roots. Purification and Reconstitution. Biomembranes Part Q, ATP-Driven Pumps and Related Transport Calcium, Proton and Potassium Pumps, vol. 157. San Diego: Academic Press.
  16. Sharma, S. C., Raj, D., Forouzandeh, M. & Bansal, M. P. ( 1996; ). Salt-induced changes in lipid composition and ethanol tolerance in Saccharomyces cerevisiae. Appl Biochem Biotechnol 56, 189–195.[CrossRef]
    [Google Scholar]
  17. Strancar, J., Sentjurc, M. & Schara, M. ( 2000; ). Fast and accurate characterization of biological membranes by EPR spectral simulations of nitroxides. J Magn Reson 142, 254–265.[CrossRef]
    [Google Scholar]
  18. Thomé, P. E. ( 2004; ). Isolation of a GPD gene from Debaryomyces hansenii encoding a glycerol 3-phosphate dehydrogenase (NAD+). Yeast 21, 119–126.[CrossRef]
    [Google Scholar]
  19. Thomé-Ortiz, P. E., Peña, A. & Ramírez, J. ( 1998; ). Monovalent cation fluxes and physiological changes of Debaryomyces hansenii grown at high concentrations of KCl and NaCl. Yeast 14, 1355–1371.[CrossRef]
    [Google Scholar]
  20. Tunblad-Johansson, I. & Adler, L. ( 1987; ). Effect of sodium chloride concentration on phospholipid fatty acid composition of yeasts differing in osmotolerance. FEMS Microbiol Lett 43, 275–278.[CrossRef]
    [Google Scholar]
  21. Tunblad-Johansson, I., Andre, L. & Adler, L. ( 1987; ). The sterol and phospholipid composition of the salt-tolerant yeast Debaryomyces hansenii grown at various concentrations of NaCl. Biochim Biophys Acta 921, 116–123.[CrossRef]
    [Google Scholar]
  22. Turk, M., Mejanelle, L., Sentjurc, M., Grimalt, J. O., Gunde-Cimerman, N. & Plemenitaš, A. ( 2004; ). Salt-induced changes in lipid composition and membrane fluidity of halophilic yeast-like melanized fungi. Extremophiles 8, 53–61.[CrossRef]
    [Google Scholar]
  23. Turk, M., Abramović, Z., Plemenitaš, A. & Gunde-Cimerman, N. ( 2007; ). Salt stress and plasma-membrane fluidity in selected extremophilic yeasts and yeast-like fungi. FEMS Yeast Res 7, 550–557.[CrossRef]
    [Google Scholar]
  24. Yoshikawa, S., Mitsui, N., Chikara, K. I., Hashimoto, H., Shimosaka, M. & Okazaki, M. ( 1995; ). Effect of salt stress on plasma membrane permeability and lipid saturation in the salt-tolerant yeast Zygosaccharomyces rouxii. J Ferment Bioeng 80, 131–135.[CrossRef]
    [Google Scholar]
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