1887

Abstract

is an opportunistic pathogen associated with contaminated powdered infant formula and a rare cause of Gram-negative sepsis that can develop into meningitis and brain abscess formation in neonates. Bacterial pathogenesis remains to be fully elucidated. In this study, the host inflammatory response was evaluated following intracranial inoculation of into infant rats. Infiltrating macrophages and neutrophils composed multiple inflammatory foci and contained phagocytosed bacteria. Several genotypically distinct strains (16S cluster groups 1–4) were shown to invade rat capillary endothelial brain cells (rBCEC4) . Further, the persistence of in macrophages varied between strains. The presence of putative genes and SOD activity may influence the survival of acidic conditions and macrophage oxidase and contribute to intracellular persistence. The influence of macrophage uptake of on immunoregulatory cytokine expression was assessed by ELISA. This demonstrated that the IL-10/IL-12 ratio is high after 24 h. This is suggestive of a type 2 immune response which is inefficient in fighting intracellular infections. These findings may help explain how the diversity in virulence traits among isolates and an unsuccessful immune response contribute to the opportunistic nature of this infection.

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2007-10-01
2020-11-24
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