1887

Abstract

is an endophytic fungus, represented by a number of isolates from tropical tree and vine species in several of the world's rainforests, that produces volatile organic compounds (VOCs) with antibiotic activity. A new isolate, E-6, of this organism, with unusual biochemical and biological properties, has been obtained from the branches of a mature (Sterculiaceae) tree growing in a dry tropical forest in SW Ecuador. This unique organism produces many VOCs not previously observed in other isolates, including butanoic acid, 2-methyl-; butanoic acid, 3-methyl-; 2-butenal, 2-methyl-; butanoic acid, 3-methylbutyl ester; 3-buten-1-ol, 3-methyl; guaiol; 1-octene, 3-ethyl-; formamide, -(1-methylpropyl); and certain azulene and naphthalene derivatives. Some compounds usually seen in other isolates also appeared in the VOCs of isolate E-6, including caryophyllene; phenylethyl alcohol; acetic acid, 2-phenylethyl ester; bulnesene; and various propanoic acid, 2-methyl- derivatives. The biological activity of the VOCs of E-6 appears different from the original isolate of this fungus, CZ-620, since a Gram-positive bacterium was killed, and and were not. Scanning electron micrographs of the mycelium of isolate E-6 showed substantial intertwining of the hyphal strands. These strands seemed to be held together by an extracellular matrix accounting for the strong mat-like nature of the mycelium, which easily lifts off the agar surface upon transfer, unlike any other isolate of this fungus. The ITS-5.8S rDNA partial sequence data showed 99 % similarity to the original strain CZ-620. For the first time, successful establishment of into its natural host, followed by recovery of the fungus, was accomplished in seedlings of . Overall, isolates of including E-6, have chemical, biological and structural characteristics that make them potentially useful in medicine, agricultural and industrial applications.

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.2007/008912-0
2007-08-01
2019-11-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/153/8/2613.html?itemId=/content/journal/micro/10.1099/mic.0.2007/008912-0&mimeType=html&fmt=ahah

References

  1. Atmosukarto, I., Castillo, U., Hess, W. M., Sears, J. & Strobel, G. ( 2005; ). Isolation and characterization of Muscodor albus I-41.3s, a volatile antibiotic producing fungus. Plant Sci 169, 854–861.[CrossRef]
    [Google Scholar]
  2. Castillo, U., Myers, S., Browne, L., Strobel, G. A., Hess, W. M., Hanks, J. & Reay, D. ( 2005; ). Scanning electron microscopy of some endophytic streptomycetes in snakevine – Kennedia nigricans. Scanning 27, 305–311.[CrossRef]
    [Google Scholar]
  3. Daisy, B. H., Gary, A., Strobel, G. A., Castillo, U., Sears, J., Weaver, D. K. & Runyon, J. B. ( 2002; ). Naphthalene production by Muscodor vitigenus, a novel endophytic fungus. Microbiology 148, 3737–3741.
    [Google Scholar]
  4. Ezra, D., Hess, W. M. & Strobel, G. A. ( 2004a; ). New endophytic isolates of Muscodor albus, a volatile antibiotic-producing fungus. Microbiology 150, 4023–4031.[CrossRef]
    [Google Scholar]
  5. Ezra, D., Jasper, J., Rogers, T., Knighton, B., Grimsrud, E. & Strobel, G. A. ( 2004b; ). Proton-transfer reaction-mass spectroscopy as a technique to measure volatile emissions of Muscodor albus. Plant Sci 166, 1471–1477.[CrossRef]
    [Google Scholar]
  6. Hoefle, G., Steglich, W. & Vorbrueggen, H. ( 1978; ). 4-Dialkyl aminopyridines as highly active acylation catalysts. Angew Chem Int Ed Engl 17, 569–583.[CrossRef]
    [Google Scholar]
  7. Mercier, J. & Jimenez, J. I. ( 2004; ). Control of decay of apples and peaches by the biofumigant fungus Muscodor albus. Postharvest Biol Technol 31, 1–8.[CrossRef]
    [Google Scholar]
  8. Reynolds, E. S. ( 1963; ). The use of lead citrate at high pH as an electron opaque stain in electron microscopy. J Cell Biol 17, 208–212.[CrossRef]
    [Google Scholar]
  9. Sopalun, K., Strobel, G. A., Hess, W. M. & Worapong, J. ( 2003; ). A record of Muscodor albus, an endophyte from Myristica fragrans, in Thailand. Mycotaxon 88, 239–247.
    [Google Scholar]
  10. Spurr, A. R. ( 1969; ). A low viscosity epoxy resin embedding medium for electron microscopy. J Ultrastruct Res 26, 31–43.[CrossRef]
    [Google Scholar]
  11. Stinson, A. M., Zidack, N. K., Strobel, G. A. & Jacobsen, B. J. ( 2003; ). Effect of mycofumigation with Muscodor albus and Muscodor roseus on seedling diseases of sugarbeet and Verticillium wilt of eggplant. Plant Dis 87, 1349–1354.[CrossRef]
    [Google Scholar]
  12. Strobel, G. A. & Daisy, B. ( 2003; ). Bioprospecting for microbial endophytes and their natural products. Microbiol Mol Biol Rev 67, 491–502.[CrossRef]
    [Google Scholar]
  13. Strobel, G. A., Dirksie, E., Sears, J. & Markworth, C. ( 2001; ). Volatile antimicrobials from Muscodor albus, a novel endophytic fungus. Microbiology 147, 2943–2950.
    [Google Scholar]
  14. Worapong, J., Strobel, G. A., Ford, E. J., Li, J. Y., Baird, G. & Hess, W. M. ( 2001; ). Muscodor albus anam. gen. et sp. nov., an endophyte from Cinnamomum zeylanicum. Mycotaxon 79, 67–79.
    [Google Scholar]
  15. Worapong, J., Strobel, G. A., Daisy, B., Castillo, U., Baird, G. & Hess, W. M. ( 2002; ). Muscodor roseus anam. sp. nov., an endophyte from Grevillea pteridifolia. Mycotaxon 81, 463–475.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.2007/008912-0
Loading
/content/journal/micro/10.1099/mic.0.2007/008912-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error