A generalized transducing phage for the murine pathogen Free

Abstract

A virulent phage (CR1) capable of generalized transduction in was isolated from the environment and characterized. is a natural pathogen of mice, causing transmissible murine colonic hyperplasia. Sequencing of its genome has recently been completed and will soon be fully annotated and published. is an important model organism for infections caused by the human pathogens enteropathogenic and enterohaemorrhagic (EPEC and EHEC). CR1 uses a lipopolysaccharide receptor, has a genome size of approximately 300 kb, and is able to transduce a variety of markers. CR1 is the first reported transducing phage for and will be a useful tool for functional genomic analysis of this important natural murine pathogen.

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2007-09-01
2024-03-28
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References

  1. Ackermann H. W. 2003; Bacteriophage observations and evolution. Res Microbiol 154:245–251
    [Google Scholar]
  2. Barthold S. W., Coleman G. L., Jacoby R. O., Livestone E. M., Jonas A. M. 1978; Transmissible murine colonic hyperplasia. Vet Pathol 15:223–236
    [Google Scholar]
  3. Demczuk W., Ahmed R., Ackermann H. W. 2004; Morphology of Salmonella enterica serovar Heidelberg typing phages. Can J Microbiol 50:873–875
    [Google Scholar]
  4. Fineran P. C., Everson L., Slater H., Salmond G. P. 2005; A GntR family transcriptional regulator (PigT) controls gluconate-mediated repression and defines a new, independent pathway for regulation of the tripyrrole antibiotic, prodigiosin, in Serratia. Microbiology 151:3833–3845
    [Google Scholar]
  5. Luperchio S. A., Schauer D. B. 2001; Molecular pathogenesis of Citrobacter rodentium and transmissible murine colonic hyperplasia. Microbes Infect 3:333–340
    [Google Scholar]
  6. Margulies M., Egholm M., Altman W. E., Attiya S., Bader J. S., Bemben L. A., Berka J., Braverman M. S., Chen Y. J. other authors 2005; Genome sequencing in microfabricated high-density picolitre reactors. Nature 437:376–380
    [Google Scholar]
  7. Mundy R., Macdonald T. T., Dougan G., Frankel G., Wiles S. 2005; Citrobacter rodentium of mice and man. Cell Microbiol 7:1697–1706
    [Google Scholar]
  8. Nataro J. P., Kaper J. B. 1998; Diarrheagenic Escherichia coli. Clin Microbiol Rev 11:142–201
    [Google Scholar]
  9. Petty N. K., Foulds I. J., Pradel E., Ewbank J. J., Salmond G. P. C. 2006; A generalized transducing phage ( φIF3) for the genomically sequenced Serratia marcescens strain Db11: a tool for functional genomics of an opportunistic human pathogen. Microbiology 152:1701–1708
    [Google Scholar]
  10. Sambrook J., Fritsch E. F., Maniatis T. A. 1989 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  11. Schauer D. B., Zabel B. A., Pedraza I. F., O'Hara C. M., Steigerwalt A. G., Brenner D. J. 1995; Genetic and biochemical characterization of Citrobacter rodentium sp. nov. J Clin Microbiol 33:2064–2068
    [Google Scholar]
  12. Smith D. S. 2005; Development of a positive selection strategy to investigate the regulation of quorum sensing in Erwinia. PhD thesis Department of Biochemistry, University of Cambridge;
    [Google Scholar]
  13. Smith M. C., Rees C. E. 1999; Exploitation of bacteriophages and their components. In Genetic Methods for Diverse Prokaryotes pp 97–132 Edited by Smith M. C., Sockett R. E. London: Academic Press;
    [Google Scholar]
  14. Wales A. D., Woodward M. J., Pearson G. R. 2005; Attaching-effacing bacteria in animals. J Comp Pathol 132:1–26
    [Google Scholar]
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