1887

Abstract

In aquatic environments, biofilms constitute an ecological niche where persists as sessile cells. However, very little information on the sessile mode of life of is currently available. We report here the development of a model biofilm of strain Lens and the first transcriptome analysis of biofilm cells. Global gene expression analysis of sessile cells as compared to two distinct populations of planktonic cells revealed that a substantial proportion of genes is differentially expressed, as 2.3 % of the 2932 predicted genes exhibited at least a twofold change in gene expression. Comparison with previous results defining the gene expression profile of replicative- and transmissive-phase suggests that sessile cells resemble bacteria in the replicative phase. Further analysis of the most strongly regulated genes in sessile cells identified two induced gene clusters. One contains genes that encode alkyl hydroperoxide reductases known to act against oxidative stress. The second encodes proteins similar to PvcA and PvcB that are involved in siderophore biosynthesis in . Since iron has been reported to modify biofilm formation in other species, we further focused on iron control of gene expression and biofilm formation. Among the genes showing the greatest differences in expression between planktonic cells and biofilm, only and were regulated by iron concentration. A Δ mutant showed no changes in biofilm formation compared to the wild-type, suggesting that the product is not mandatory for biofilm formation. However, biofilm formation by wild-type and a Δ strain was clearly inhibited in iron-rich conditions.

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2008-01-01
2019-10-23
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Genes with significant ( <0.05) fold change in expression greater than 2 in at least one sessile versus planktonic cells comparison [ PDF] (100 kb) Expression fold change of genes associated with iron homeostasis [ PDF] (11 kb)

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Genes with significant ( <0.05) fold change in expression greater than 2 in at least one sessile versus planktonic cells comparison [ PDF] (100 kb) Expression fold change of genes associated with iron homeostasis [ PDF] (11 kb)

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