1887

Abstract

The purpose of this study was to evaluate a multilocus sequence typing (MLST) scheme for intestinal spirochaetes of the genus . Eight loci mainly coding for enzymes previously used in multilocus enzyme electrophoresis analysis of species were examined in 66 field isolates and type/reference strains. The isolates and strains were recovered from pigs, birds, dogs and a mouse and originated from seven European countries, the USA and Canada. Forty-six isolates represented recognized species and 20 represented provisionally designated species or isolates that have not been classified. Only two loci gave PCR products for all 66 strains and isolates, but amplicons for seven loci were obtained for 44 of the isolates. Sequences for each locus had a DNA allelic variation of 30–47 and an amino acid allelic variation of 14–47 that gave rise to the same number of sequence and amino acid types (58) for the strains and isolates studied. A population snapshot based on sequence and amino acid types showed a close phylogenetic relationship amongst the porcine isolates from the same geographical regions, and indicated a close evolutionary relationship between isolates recovered from pigs and mallards. A general concordance was obtained between the MLST groupings and classifications based on culture and biochemical tests, 16S rDNA sequence analysis and random amplified polymorphic DNA analysis. This is a first step towards establishing an MLST system for use in identifying species and determining relationships between individual strains and species in the genus.

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2007-12-01
2024-12-07
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References

  1. Atyeo R. F., Oxberry S. L., Hampson D. J. 1996; Pulsed-field gel electrophoresis for sub-specific differentiation of Serpulina pilosicoli (formerly ‘ Anguillina coli ’. FEMS Microbiol Lett 141:77–81
    [Google Scholar]
  2. Atyeo R. F., Oxberry S. L., Hampson D. J. 1999a; Analysis of Serpulina hyodysenteriae strain variation and its molecular epidemiology using pulsed-field gel electrophoresis. Epidemiol Infect 123:133–138
    [Google Scholar]
  3. Atyeo R. F., Stanton T. B., Jensen N. S., Suriyaarachichi D. S., Hampson D. J. 1999b; Differentiation of Serpulina species by NADH oxidase gene ( nox ) sequence comparisons and nox-based polymerase chain reaction tests. Vet Microbiol 67:47–60
    [Google Scholar]
  4. Diavatopoulos D. A., Cummings C. A., Schouls L. M., Brinig M. M., Relman D. A., Mooi F. R. 2005; Bordetella pertussis , the causative agent of whooping cough, evolved from a distinct, human-associated lineage of B. bronchiseptica . PLoS Pathogens 1:e45
    [Google Scholar]
  5. Didelot X., Falush D. 2006; Inference of bacterial microevolution using multilocus sequence data. Genetics 175:1251–1266
    [Google Scholar]
  6. Dugourd D., Jacques M., Bigras-Poulin M., Harel J. 1996; Characterization of Serpulina hyodysenteriae isolates of serotypes 8 and 9 by random amplification of polymorphic DNA analysis. Vet Microbiol 48:305–314
    [Google Scholar]
  7. Duhamel G. E. 2001; Comparative pathology and pathogenesis of naturally acquired and experimentally induced colonic spirochetosis. Anim Health Res Rev 2:3–17
    [Google Scholar]
  8. Duhamel G. E., Trott D. J., Muniappa N., Mathiesen M. R., Tarasiuk K., Lee J. I., Hampson D. J. 1998; Canine intestinal spirochetes consist of Serpulina pilosicoli and a newly identified group provisionally designated “ Serpulina canis ” sp. nov. J Clin Microbiol 36:2264–2270
    [Google Scholar]
  9. Feil E. J., Li B. C., Aanensen D. M., Hanage W. P., Spratt B. G. 2004; eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol 186:1518–1530
    [Google Scholar]
  10. Fellström $., Gunnarsson A. 1995; Phenotypical characterisation of intestinal spirochaetes isolated from pigs. Res Vet Sci 59:1–4
    [Google Scholar]
  11. Fellström C., Holmgren N. 2005; Mice as reservoirs for swine dysentery in a fattening herd. Svensk Veterinartidning 57:19–21
    [Google Scholar]
  12. Fellström C., Pettersson B., Uhlén M., Gunnarsson A., Johansson K.-E. 1995; Phylogeny of Serpulina based on sequence analyses of the 16S rRNA gene and comparison with a scheme involving biochemical classification. Res Vet Sci 59:5–9
    [Google Scholar]
  13. Fellström C., Karlsson M., Pettersson B., Zimmerman U., Gunnarsson A., Aspan A. 1999; Emended descriptions of indole negative and indole positive isolates of Brachyspira ( Serpulina ) hyodysenteriae . Vet Microbiol 70:225–238
    [Google Scholar]
  14. Fransson T., Pettersson J. 2001; Mallard Anas platyrhynchos . In Swedish Bird Ringing Atlas vol. 1 pp 96–101 Stockholm: Naturhistoriska Riksmuseet & Sveriges Ornitologiska Förening;
    [Google Scholar]
  15. Hampson D. J., Stanton T. B. 1997 Intestinal Spirochaetes in Domestic Animals and Humans Wallingford, UK: CAB International;
  16. Hampson D. J., Combs B. G., Harders S. J., Connaughton I. D., Fahy V. A. 1991; Isolation of Treponema hyodysenteriae from a wild rat living on a piggery. Aust Vet J 68:308
    [Google Scholar]
  17. Harris D. L., Glock R. D., Christensen C. R., Kinyon J. M. 1972; Inoculation of pigs with Treponema hyodysenteriae (new species) and reproduction f the disease. Vet Med Small Anim Clin 67:61–64
    [Google Scholar]
  18. Hovind-Hougen K., Birch-Andersen A., Henrik-Nielsen R., Orholm M., Pedersen J. O., Teglbjaerg P. S., Thaysen E. H. 1982; Intestinal spirochetosis: morphological characterization and cultivation of the spirochete Brachyspira aalborgi gen. nov. sp. nov. J Clin Microbiol 16:1127–1136
    [Google Scholar]
  19. Jansson D. S., Johansson K.-E., Olofsson T., Råsbäck T., Vågsholm I., Pettersson B., Gunnarsson A., Fellström C. 2004; Brachyspira hyodysenteriae and other strongly beta-haemolytic and indole-positive spirochaetes isolated from mallards ( Anas platyrhynchos . J Med Microbiol 53:293–300
    [Google Scholar]
  20. Joens L. A. 1980; Experimental transmission of Treponema hyodysenteriae from mice to pigs. Am J Vet Res 41:1225–1226
    [Google Scholar]
  21. Johansson K.-E., Duhamel G. E., Bergsjø B., Engvall E. O., Persson M., Pettersson B., Fellström C. 2004; Identification of three clusters of canine intestinal spirochaetes by biochemical and 16S rDNA sequence analysis. J Med Microbiol 53:345–350
    [Google Scholar]
  22. Karlsson M., Aspán A., Landén A., Franklin A. 2004; Further characterization of porcine Brachyspira hyodysenteriae isolates with decreased susceptibility to tiamulin. J Med Microbiol 53:281–285
    [Google Scholar]
  23. Kinyon J. M., Harris D.L. 1979; Treponema innocens , a new species of intestinal bacteria, and emended description of the type strain of Treponema hyodysenteriae Harris et al . Int J Syst Bacteriol 29:102–109
    [Google Scholar]
  24. Kinyon J. M., Harris D. L., Glock R. D. 1977; Enteropathogenicity of various isolates of Treponema hyodysenteriae . Infect Immun 15:638–646
    [Google Scholar]
  25. Kumar S., Tamura K., Jakobsen I. B., Nei M. 2001; mega2: molecular evolutionary genetics analysis software. Bioinformatics 17:1244–1245
    [Google Scholar]
  26. Lee J. I., Hampson D. J., Lymbery A. J., Harders S. J. 1993; The porcine intestinal spirochaetes: identification of new genetic groups. Vet Microbiol 34:273–285
    [Google Scholar]
  27. Ludwig W., Klenk H.-P. 2001; Overview: a phylogenetic backbone and taxonomic framework for procaryotic systematics. In Bergey's Manual of Systematic Bacteriology pp 49–65 Edited by Garrity G. New York: Springer-Verlag;
    [Google Scholar]
  28. Maiden M. C., Bygraves J. A., Feil E., Morelli G., Russell J. E., Urwin R., Zhang Q., Zhou J., Zurth K. other authors 1998; Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci U S A 95:3140–3145
    [Google Scholar]
  29. McLaren A. J., Trott D. J., Swayne D. E., Oxberry S. L., Hampson D. J. 1997; Genetic and phenotypic characterization of intestinal spirochetes colonizing chickens and allocation of known pathogenic isolates to three distinct genetic groups. J Clin Microbiol 35:412–417
    [Google Scholar]
  30. Miller W. G., On S. L., Wang G., Fontanoz S., Lastovica A. J., Mandrell R. E. 2005; Extended multilocus sequence typing system for Campylobacter coli , C. lari , C. upsaliensis , and C. helveticus . J Clin Microbiol 43:2315–2329
    [Google Scholar]
  31. Pettersson B., Fellström C., Andersson A., Uhlén M., Gunnarsson A., Johansson K.-E. 1996; The phylogeny of intestinal porcine spirochetes ( Serpulina species) based on sequence analysis of the 16S rRNA gene. J Bacteriol 178:4189–4199
    [Google Scholar]
  32. Pringle M., Poehlsgaard J., Vester B., Long K. S. 2004; Mutations in ribosomal protein L3 and 23S ribosomal RNA at the peptidyl transferase centre are associated with reduced susceptibility to tiamulin in Brachyspira spp. isolates. Mol Microbiol 54:1295–1306
    [Google Scholar]
  33. Pringle M., Landen A., Franklin A. 2006; Tiamulin resistance in porcine Brachyspira pilosicoli isolates. Res Vet Sci 80:1–4
    [Google Scholar]
  34. Quednau M., Ahrne S., Petersson A. C., Molin G. 1998; Identification of clinically important species of Enterococcus within 1 day with randomly amplified polymorphic DNA (RAPD. Curr Microbiol 36:332–336
    [Google Scholar]
  35. Råsbäck T., Fellström C., Bergsjø B., Cizek A., Collin K., Gunnarsson A., Jensen S. M., Mars A., Thomson J. other authors 2005; Assessment of diagnostics and antimicrobial susceptibility testing of Brachyspira species using a ring test. Vet Microbiol 109:229–243
    [Google Scholar]
  36. Råsbäck T., Fellström C., Gunnarsson A., Aspán A. 2006; Comparison of culture and biochemical tests with PCR for detection of Brachyspira hyodysenteriae and Brachyspira pilosicoli . J Microbiol Methods 66:347–353
    [Google Scholar]
  37. Råsbäck T., Jansson S. D., Johansson K.-E., Fellström C. 2007; A novel enteropathogenic, strongly haemolytic spirochaete isolated from pig and mallard, provisionally designated ‘ Brachyspira suanatina ’ sp. nov. Environ Microbiol 9:983–991
    [Google Scholar]
  38. Rozen S., Skaletsky H. 2000; Primer3 on the WWW for general users and for biologist programmers. Methods Mol Biol 132:365–386
    [Google Scholar]
  39. Songer J. G., Glock R. D., Schwartz K. J., Harris D. L. 1978; Isolation of Treponema hyodysenteriae from sources other than swine. J Am Vet Med Assoc 172:464–466
    [Google Scholar]
  40. Stanton T. B., Trott D. J., Lee J. I., McLaren A. J., Hampson D. J., Paster B. J., Jensen N. S. 1996; Differentiation of intestinal spirochaetes by multilocus enzyme electrophoresis analysis and 16S rRNA sequence comparisons. FEMS Microbiol Lett 136:181–186
    [Google Scholar]
  41. Stanton T. B., Fournie-Amazouz E., Postic D., Trott D. J., Grimont P. A., Baranton G., Hampson D. J., Saint Girons I. 1997; Recognition of two new species of intestinal spirochetes: Serpulina intermedia sp.nov. and Serpulina murdochii sp. nov. Int J Syst Bacteriol 47:1007–1012
    [Google Scholar]
  42. Stanton T. B., Postic D., Jensen N. S. 1998; Serpulina alvinipulli sp. nov., a new Serpulina species that is enteropathogenic for chickens. Int J Syst Bacteriol 48:669–676
    [Google Scholar]
  43. Stephens C. P., Hampson D. J. 2001; Intestinal spirochete infections of chickens: a review of disease associations, epidemiology and control. Anim Health Res Rev 2:83–91
    [Google Scholar]
  44. Suriyaarachchi D. S., Mikosza A. S., Atyeo R. F., Hampson D. J. 2000; Evaluation of a 23S rDNA polymerase chain reaction assay for identification of Serpulina intermedia , and strain typing using pulsed-field gel electrophoresis. Vet Microbiol 71:139–148
    [Google Scholar]
  45. Taylor D. J., Alexander T. J. 1971; The production of dysentery in swine by feeding cultures containing a spirochaete. Br Vet J 127:58–61
    [Google Scholar]
  46. Taylor D. J., Simmons J. R., Laird H. M. 1980; Production of diarrhoea and dysentery in pigs by feeding pure cultures of a spirochaete differing from Treponema hyodysenteriae . Vet Rec 106:326–332
    [Google Scholar]
  47. Thomson J. R., Smith W. J., Murray B. P., Murray D., Dick J. E., Sumption K. J. 2001; Porcine enteric spirochete infections in the UK: surveillance data and preliminary investigation of atypical isolates. Anim Health Res Rev 2:31–36
    [Google Scholar]
  48. Torriani S., Zapparoli G., Dellaglio F. 1999; Use of PCR-based methods for rapid differentiation of Lactobacillus delbrueckii subsp. bulgaricus and L.delbrueckii subsp. lactis . Appl Environ Microbiol 65:4351–4356
    [Google Scholar]
  49. Trott D. J., Atyeo R. F., Lee J. I., Swayne D. A., Stoutenburg J. W., Hampson D. J. 1996a; Genetic relatedness amongst intestinal spirochaetes isolated from rats and birds. Lett Appl Microbiol 23:431–436
    [Google Scholar]
  50. Trott D. J., Stanton T. B., Jensen N. S., Duhamel G. E., Johnson J. L., Hampson D. J. 1996b; Serpulina pilosicoli sp. nov., the agent of porcine intestinal spirochetosis. Int J Syst Bacteriol 46:206–215
    [Google Scholar]
  51. Urwin R., Maiden M. C. 2003; Multi-locus sequence typing: a tool for global epidemiology. Trends Microbiol 11:479–487
    [Google Scholar]
  52. Ventura M., Canchaya C., Del Casale A., Dellaglio F., Neviani E., Fitzgerald G. F., van Sinderen D. 2006; Analysis of bifidobacterial evolution using a multilocus approach. Int J Syst Evol Microbiol 56:2783–2792
    [Google Scholar]
  53. Woese C. R. 1987; Bacterial evolution. Microbiol Rev 51:221–271
    [Google Scholar]
  54. Zuerner R. L., Stanton T. B. 1994; Physical and genetic map of the Serpulina hyodysenteriae B78T chromosome. J Bacteriol 176:1087–1092
    [Google Scholar]
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