1887

Abstract

The -dependent transcriptional regulator SfnR is essential for the use of dimethyl sulfone (DMSO) as a sulfur source by DS1. SfnR binds three SfnR-binding sites (sites 1, 2 and 3) within an intergenic region of the divergently transcribed and gene clusters. The site 1 region, proximal to the gene, is indispensable for the expression of the operon, which encodes components of DMSO monooxygenase. We investigated the transcriptional regulation of the operon and possible functions of the gene. RT-PCR analysis revealed that the gene cluster, which is similar to homologues of the acyl-CoA dehydrogenase family, was transcribed as an operon, and its expression was regulated by SfnR under conditions of sulfate starvation. Deletion analyses using as a reporter demonstrated that the region up to at least −138 bp from the transcription start point of (containing sites 2 and 3) was necessary for the expression of the operon. A growth test of the -disrupted mutant revealed the possibility that may be involved in the use of methanethiol as a sulfur source.

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2007-09-01
2020-09-27
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