1887

Abstract

Haem is a unique tetrapyrrole molecule that serves as a prosthetic group of cytochrome , which reduces nitrite to nitric oxide during the process of denitrification. Very little information is available regarding the biosynthesis of haem . The extreme difficulty in studying the haem biosynthetic pathway can be partly attributed to the lack of a theoretical basis for experimental investigation. We report here a gene cluster encoding enzymes involved in the biosynthesis of haem in two heliobacterial species, and . The gene organization of the cluster is conserved between the two species, and contains a complete set of genes that lead to the biosynthesis of uroporphyrinogen III and genes thought to be involved in the late steps of haem biosynthesis. Detailed bioinformatics analysis of some of the proteins encoded in the gene cluster revealed important clues to the precise biochemical roles of the proteins in the biosynthesis of haem , as well as the membrane transport and insertion of haem into an apocytochrome during the maturation of cytochrome .

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2007-10-01
2019-10-23
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