1887

Abstract

is an important pathogen of maize that causes ear rot and produces the mycotoxins known as fumonisins. To date, knowledge of pathogenicity and the regulation of fumonisin biosynthesis in is limited. Here, the molecular characterization of , a gene encoding a putative 540 aa protein that belongs to a glycolipid-anchored surface (GAS) protein family, is presented. was identified as an expressed sequence tag (EST) upregulated in a culture condition conducive to conidiation and fumonisin B (FB) production. null mutants GAM126 (Δ : : ) and GAG8 (Δ : : ) exhibited restricted growth, with more aerial hyphae than their wild-type progenitor on solid media. No defect in mycelial mass or filamentous growth was observed when the GAM126 and GAG8 strains were grown in liquid media under shaking conditions. When grown in suspended conditions, GAM126 and GAG8 strains produced significantly fewer conidia and produced comparatively densely branched hyphae. Concanavalin A staining indicated that the deletion altered the cell wall carbohydrate composition/deposition process. Deletion of did not affect the production level of FB or virulence on maize seedlings and stalks. Complementation of GAM126 with the wild-type gene restored growth, conidiation and cell wall abnormality phenotypes. The results suggest that is associated with growth, development and conidiation in , but not with pathogenicity or regulation of FB.

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2007-09-01
2020-08-15
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References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res25:3389–3402
    [Google Scholar]
  2. Bickle M., Delley P. A., Schmidt A., Hall M. N.. 1998; Cell wall integrity modulates RHO1 activity via the exchange factor ROM2. EMBO J17:2235–2245
    [Google Scholar]
  3. Brown D. W., Butchko R. A., Busman M., Proctor R.. 2007; The Fusarium verticillioides FUM gene cluster encodes a Zn(II)2Cys6 protein that affects FUM gene expression and fumonisin production. Eukaryot Cell (in press)
    [Google Scholar]
  4. Caracuel Z., Martinez-Rocha A. L., Di Pietro A., Madrid M. P., Roncero M. I.. 2005; Fusarium oxysporum gas1 encodes a putative beta-1,3-glucanosyltransferase required for virulence on tomato plants. Mol Plant Microbe Interact18:1140–1147
    [Google Scholar]
  5. Chabane S., Sarfati J., Ibrahim-Granet O., Du C., Schmidt C., Mouyna I., Prevost M. C., Calderone R., Latge J. P.. 2006; Glycosylphosphatidylinositol-anchored Ecm33p influences conidial cell wall biosynthesis in Aspergillus fumigatus. Appl Environ Microbiol72:3259–3267
    [Google Scholar]
  6. Davydenko S. G., Feng D., Jantti J., Keranen S.. 2005; Characterization of GPI14/ YJR013w mutation that induces the cell wall integrity signalling pathway and results in increased protein production in Saccharomyces cerevisiae. Yeast22:993–1009
    [Google Scholar]
  7. De Bernardis F., Muhlschlegel F. A., Cassone A., Fonzi W. A.. 1998; The pH of the host niche controls gene expression in and virulence of Candida albicans. Infect Immun66:3317–3325
    [Google Scholar]
  8. De Groot P. W. J., Ram A. F., Klis F. M.. 2005; Features and functions of covalently linked proteins in fungal cell walls. Fungal Genet Biol42:657–675
    [Google Scholar]
  9. Dodd J. L.. 1980; The role of plant stresses in development of corn stalk rots. Plant Dis64:533–537
    [Google Scholar]
  10. Flaherty J. E., Pirttila A. M., Bluhm B. H., Woloshuk C. P.. 2003; PAC1, a pH-regulatory gene from Fusarium verticillioides. Appl Environ Microbiol69:5222–5227
    [Google Scholar]
  11. Ghannoum M. A., Spellberg B., Saporito-Irwin S. M., Fonzi W. A.. 1995; Reduced virulence of Candida albicans PHR1 mutants. Infect Immun63:4528–4530
    [Google Scholar]
  12. Imai K., Noda Y., Adachi H., Yoda K.. 2005; A novel endoplasmic reticulum membrane protein Rcr1 regulates chitin deposition in the cell wall of Saccharomyces cerevisiae. J Biol Chem280:8275–8284
    [Google Scholar]
  13. Kapteyn J. C., Montijn R. C., Vink E., De la Cruz J., Llobell A., Douwes J. E., Shimoi H., Lipke P. N., Klis F. M.. 1996; Retention of Saccharomyces cerevisiae cell wall proteins through a phosphodiester-linked β-1,3-/ β-1,6-glucan heteropolymer. Glycobiology6:337–345
    [Google Scholar]
  14. Kapteyn J. C., Ram A. F., Groos E. M., Kollar R., Montijn R. C., Van den Ende H., Llobell A., Cabib E., Klis F. M.. 1997; Altered extent of cross-linking of β1,6-glucosylated mannoproteins to chitin in Saccharomyces cerevisiae mutants with reduced cell wall β1,3-glucan content. J Bacteriol179:6279–6284
    [Google Scholar]
  15. Kollar R., Reinhold B. B., Petrakova E., Yeh H. J., Ashwell G., Drgonova J., Kapteyn J. C., Klis F. M., Cabib E.. 1997; Architecture of the yeast cell wall. β(1 → 6)-glucan interconnects mannoprotein, β(1 → 3)-glucan, and chitin. J Biol Chem272:17762–17775
    [Google Scholar]
  16. Kottom T. J., Thomas C. F. Jr, Limper A. H.. 2001; Characterization of Pneumocystis carinii PHR1, a pH-regulated gene important for cell wall integrity. J Bacteriol183:6740–6745
    [Google Scholar]
  17. Madaule P., Axel R., Myers A. M.. 1987; Characterization of two members of the rho gene family from the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A84:779–783
    [Google Scholar]
  18. Marasas W. F. O.. 2001; Discovery and occurrence of the fumonisins: a historical perspective. Environ Health Perspect109:239–243
    [Google Scholar]
  19. Marchler-Bauer A., Anderson J. B., Cherukuri P. F., DeWeese-Scott C., Geer L. Y., Gwadz M., He S., Hurwitz D. I., Jackson J. D.. other authors 2005; CDD: a Conserved Domain Database for protein classification. Nucleic Acids Res33:D192–D196
    [Google Scholar]
  20. Martinez-Lopez R., Monteoliva L., Ez-Orejas R., Nombela C., Gil C.. 2004; The GPI-anchored protein CaEcm33p is required for cell wall integrity, morphogenesis and virulence in Candida albicans. Microbiology150:3341–3354
    [Google Scholar]
  21. Michaelson M. E.. 1957; Factors affecting development of stalk rots of corn caused by Diplodia zeae and Gibberella zeae. Phytopathology47:499–503
    [Google Scholar]
  22. Mouyna I., Fontaine T., Vai M., Monod M., Fonzi W. A., Diaquin M., Popolo L., Hartland R. P., Latge J. P.. 2000; Glycosylphosphatidylinositol-anchored glucanosyltransferases play an active role in the biosynthesis of the fungal cell wall. J Biol Chem275:14882–14889
    [Google Scholar]
  23. Mouyna I., Morelle W., Vai M., Monod M., Lechenne B., Fontaine T., Beauvais A., Sarfati J., Prevost M. C.. other authors 2005; Deletion of GEL2 encoding for a beta(1–3)glucanosyltransferase affects morphogenesis and virulence in Aspergillus fumigatus. Mol Microbiol56:1675–1688
    [Google Scholar]
  24. Muhlschlegel F. A., Fonzi W. A.. 1997; PHR2 of Candida albicans encodes a functional homolog of the pH-regulated gene PHR1 with an inverted pattern of pH-dependent expression. Mol Cell Biol17:5960–5967
    [Google Scholar]
  25. Munkvold G. P., Desjardins A. E.. 1997; Fumonisins in maize: can we reduce their occurrence?. Plant Dis81:556–565
    [Google Scholar]
  26. Nelson P. E., Desjardins A. E., Plattner R. D.. 1993; Fumonisins: mycotoxins produced by fusarium species: biology, chemistry, and significance. Annu Rev Phytopathol31:233–252
    [Google Scholar]
  27. Pirttilä A. M., McIntyre L. M., Payne G. A., Woloshuk C. P.. 2004; Expression profile analysis of wild-type and fcc1 mutant strains of Fusarium verticillioides during fumonisin biosynthesis. Fungal Genet Biol41:647–656
    [Google Scholar]
  28. Popolo L., Vai M., Gatti E., Porello S., Bonfante P., Balestrini R., Alberghina L.. 1993; Physiological analysis of mutants indicates involvement of the Saccharomyces cerevisiae GPI-anchored protein gp115 in morphogenesis and cell separation. J Bacteriol175:1879–1885
    [Google Scholar]
  29. Porta A., Ramon A. M., Fonzi W. A.. 1999; PRR1, a homolog of Aspergillus nidulans palF, controls pH-dependent gene expression and filamentation in Candida albicans. J Bacteriol181:7516–7523
    [Google Scholar]
  30. Proctor R. H., Desjardins A. E., Plattner R. D., Hohn T. M.. 1999; A polyketide synthase gene required for biosynthesis of fumonisin mycotoxins in Gibberella fujikuroi mating population A. Fungal Genet Biol27:100–112
    [Google Scholar]
  31. Proctor R. H., Brown D. W., Plattner R. D., Desjardins A. E.. 2003; Co-expression of 15 contiguous genes delineates a fumonisin biosynthetic gene cluster in Gibberella moniliformis. Fungal Genet Biol38:237–249
    [Google Scholar]
  32. Ram A. F. J., Klis F. M.. 2006; Identification of fungal cell wall mutants using susceptibility assays based on Calcofluor white and Congo red. Nat Protoc1:2253–2256
    [Google Scholar]
  33. Ram A. F., Wolters A., Ten Hoopen R., Klis F. M.. 1994; A new approach for isolating cell wall mutants in Saccharomyces cerevisiae by screening for hypersensitivity to calcofluor white. Yeast10:1019–1030
    [Google Scholar]
  34. Roncero C., Valdivieso M. H., Ribas J. C., Duran A.. 1988; Isolation and characterization of Saccharomyces cerevisiae mutants resistant to calcofluor white. J Bacteriol170:1950–1954
    [Google Scholar]
  35. Sagaram U. S., Kolomiets M., Shim W. B.. 2006; Regulation of fumonisin biosynthesis in Fusarium verticillioides-maize system. Plant Pathol J22:203–210
    [Google Scholar]
  36. Sambrook J., Russell D. W.. 2001; Molecular Cloning: a Laboratory Manual, 3rd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  37. Saporito-Irwin S. M., Birse C. E., Sypherd P. S., Fonzi W. A.. 1995; PHR1, a pH-regulated gene of Candida albicans, is required for morphogenesis. Mol Cell Biol15:601–613
    [Google Scholar]
  38. Shaw B. D., Hoch H. C.. 1999; The pycnidiospore of Phyllosticta ampelicida: surface properties involved in substratum attachment and germination. Mycol Res103:915–924
    [Google Scholar]
  39. Shim W. B., Woloshuk C. P.. 1999; Nitrogen repression of fumonisin B1 biosynthesis in Gibberella fujikuroi. FEMS Microbiol Lett177:109–116
    [Google Scholar]
  40. Shim W. B., Woloshuk C. P.. 2001; Regulation of fumonisin B1 biosynthesis and conidiation in Fusarium verticillioides by a cyclin-like (C-type) gene, FCC1. Appl Environ Microbiol67:1607–1612
    [Google Scholar]
  41. Shim W. B., Sagaram U. S., Choi Y. E., So J., Wilkinson H. H., Lee Y. W.. 2006; FSR1 is essential for virulence and female fertility in Fusarium verticillioides and F. graminearum. Mol Plant Microbe Interact19:725–733
    [Google Scholar]
  42. Smith E. E., Goldstein I. J.. 1967; Protein–carbohydrate interaction. V. Further inhibition studies directed toward defining the stererochemical requirements of the reactive sites of concanavalin A. Arch Biochem Biophys121:88–95
    [Google Scholar]
  43. Tilburn J., Sarkar S., Widdick D. A., Espeso E. A., Orejas M., Mungroo J., Penalva M. A., Arst H. N.. 1995; The Aspergillus Pacc zinc-finger transcription factor mediates regulation of both acid-expressed and alkaline-expressed genes by ambient pH. EMBO J14:779–790
    [Google Scholar]
  44. Upadhyay S., Shaw B. D.. 2006; A phosphoglucose isomerase mutant in Aspergillus nidulans is defective in hyphal polarity and conidiation. Fungal Genet Biol43:739–751
    [Google Scholar]
  45. White D. G.. 1999; Compendium of Corn Disease, 3rd edn. St Paul, MN: APS Press;
  46. Yu J. H., Hamari Z., Han K. H., Seo J. A., Reyes-Dominguez Y., Scazzocchio C.. 2004; Double-joint PCR: a PCR-based molecular tool for gene manipulations in filamentous fungi. Fungal Genet Biol41:973–981
    [Google Scholar]
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