1887

Abstract

is an important pathogen of maize that causes ear rot and produces the mycotoxins known as fumonisins. To date, knowledge of pathogenicity and the regulation of fumonisin biosynthesis in is limited. Here, the molecular characterization of , a gene encoding a putative 540 aa protein that belongs to a glycolipid-anchored surface (GAS) protein family, is presented. was identified as an expressed sequence tag (EST) upregulated in a culture condition conducive to conidiation and fumonisin B (FB) production. null mutants GAM126 (Δ : : ) and GAG8 (Δ : : ) exhibited restricted growth, with more aerial hyphae than their wild-type progenitor on solid media. No defect in mycelial mass or filamentous growth was observed when the GAM126 and GAG8 strains were grown in liquid media under shaking conditions. When grown in suspended conditions, GAM126 and GAG8 strains produced significantly fewer conidia and produced comparatively densely branched hyphae. Concanavalin A staining indicated that the deletion altered the cell wall carbohydrate composition/deposition process. Deletion of did not affect the production level of FB or virulence on maize seedlings and stalks. Complementation of GAM126 with the wild-type gene restored growth, conidiation and cell wall abnormality phenotypes. The results suggest that is associated with growth, development and conidiation in , but not with pathogenicity or regulation of FB.

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2007-09-01
2019-11-18
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