1887

Abstract

Most of the spontaneous poly--glutamate (-PGA)-deficient mutants of () appear to have resulted from the insertion of IS exclusively into the gene. However, complete genomic analysis of 168 a close relative of (), revealed no IS insertion. Preliminary experiments using a transformable ‘’ strain indicated that the frequency of transposition of IS was exceptionally high under competence-developing conditions. On the other hand, such high-frequency transposition was not observed when cells were grown in a rich medium, such as LB medium, suggesting that there must be suitable environmental conditions that give rise to the transposition of IS. To assess the behaviour of IS and explore any host factors playing roles in IS transposition, an intermolecular transposition assay system was constructed using a modified IS element in 168. Here, the details of the intermolecular transposition assay system are given, and the increase in transposition frequency observed under high-temperature and competence-inducing conditions is described.

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2007-08-01
2019-10-13
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