1887

Abstract

The Gram-negative proteobacterium can survive and multiply within a variety of eukaryotic cells, including macrophages. This property is believed to be important for its ability to cause the disease melioidosis in a wide range of animal species, including humans. To identify determinants that are important for the ability of to survive within macrophages, expression technology (IVET) was employed. Several putative macrophage-inducible genes were identified that are likely to contribute to the virulence of , including three genes (-5, -5 and -5) located within the same type VI secretion system cluster (-5), , encoding a natural resistance-associated macrophage protein (NRAMP)-like manganese ion transporter, and a haem acquisition gene, . The macrophage-inducibility of the -5 gene cluster was confirmed by reporter gene analysis. Construction of -5 and null mutants indicated that expression of the -5 unit and the operon were not required for intramacrophage survival. A further five units were identified within the genome that, together with -5, account for approximately 2.3 % of the total genome size. The presence of six type VI secretion systems in this organism is likely to be an important factor in making this bacterium such a versatile pathogen.

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2007-08-01
2020-08-09
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