RT Journal Article SR Electronic(1) A1 Berghoff, Julia A1 Viezens, Juliane A1 Guptill, Lynn A1 Fabbi, Massimo A1 Arvand, MardjanYR 2007 T1 Bartonella henselae exists as a mosaic of different genetic variants in the infected host JF Microbiology, VO 153 IS 7 SP 2045 OP 2051 DO https://doi.org/10.1099/mic.0.2007/006379-0 PB Microbiology Society, SN 1465-2080, AB Bartonella henselae is a fastidious bacterium associated with infections in humans and cats. The mechanisms involved in the long-term survival of bartonellae despite vigorous host immune responses are poorly understood. Generation of genetic variants is a possible strategy to circumvent the host specific immune responses. The authors have recently demonstrated the coexistence of different genetic variants within the progeny of three primary B. henselae isolates from Berlin by PFGE analysis. Aims of the present study were to determine whether coexistence of different variants is a common feature of B. henselae isolates worldwide and whether the genetic variants originally emerged in vivo. Thirty-four primary isolates from different geographical regions were analysed by subjecting multiple single-colony-derived cultures to PFGE analysis. Up to three genetic variants were detected within 20 (58.8 %) isolates, indicating that most primary isolates display a mosaic-like structure. The close relatedness of the genetic variants within an isolate was confirmed by multi-locus sequence typing. In contrast to the primary isolates, no genetic variants were detected within the progeny of 20 experimental clones generated in vitro from 20 primary isolates, suggesting that the variants were not induced in vitro during the procedure of PFGE analysis. Hence, the genetic variants within a primary isolate most likely originally emerged in vivo. Consideration of the mosaic structure of primary isolates is essential when interpreting typing studies on B. henselae., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.2007/006379-0