1887

Abstract

Four independent nonsense mutations were engineered into the chromosomal gene, and reversion rates back to LacZ phenotypes were determined. The mutation potential of bases within putative DNA secondary structures formed during transcription was predicted by a sliding-window analysis that simulates successive folding of the ssDNA creating these structures. The relative base mutabilities predicted by the computer program correlated with experimentally determined reversion rates in three of the four mutants analysed. The nucleotide changes in revertants at one nonsense codon site consisted of a triple mutation, presumed to occur by a templated repair mechanism. Additionally, the effect of supercoiling on mutation was investigated and, in general, reversion rates increased with higher levels of negative supercoiling. Evidence indicates that predicted secondary structures are in fact formed and that directed mutation in response to starvation stress is dependent upon the exposure of particular bases, the stability of the structures in which these bases are unpaired and the level of DNA supercoiling within the cell.

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2007-07-01
2019-10-14
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vol. , part 7, pp. 2180-2189

Comparison of mutation rates for each supercoiling strain and mutant over time. (a) LacZ1; (b) LacZ2; (c) LacZ3; (d) LacZ4. NB, ordinate axis scales vary.



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