1887

Abstract

cells growing in medium containing sugars accumulate glycogen in the early exponential-growth phase, and start to degrade this polymer at entry into the stationary phase. In a first attempt to investigate glycogen degradation, the gene, which encodes a protein with 46 % identity to the isoamylase-type debranching enzyme of , was analysed, expressed and inactivated The purified gene product showed debranching activity towards glycogen, amylopectin and starch. Chromosomal inactivation of in wild-type led to slower growth and to a higher intracellular glycogen pool throughout growth, when compared to those in the parental strain. This result suggests that glycogen synthesis and degradation occur simultaneously in . When exposed to hyperosmotic shock, rapidly degrades glycogen, and at the same time, synthesizes the osmoprotectant trehalose. The mutant, however, synthesized only minor amounts of trehalose throughout cultivation, and its growth ceased after hyperosmotic shock. Taken together, the results indicate that the gene product is essential for glycogen degradation in , that glycogen is constantly recycled in , and that it serves as a carbon store for trehalose synthesis via the TreYZ pathway after hyperosmotic shock.

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2007-07-01
2020-04-09
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