1887

Abstract

is the pathogen most commonly associated with morbidity and mortality in cystic fibrosis (CF) patients. The host–pathogen interactions responsible for progressive CF lung diseases are complex. However, there is growing interest in the role of hypermutable (that is, those strains with an increased mutation frequency due to mutations in mismatch repair and error prevention genes), in terms of both bacterial adaptation and antimicrobial resistance. The prevalence of hypermutable in chronic CF infection has been established, and at 37 % is surprisingly high. To the authors’ knowledge, there are no reports of prevalence during the early stages of infection, in environmental pseudomonas, which are believed to be the primary source of infection, and in epidemic strains, which have emerged as a major challenge. The aim of this study was to establish the prevalence of hypermutable in these pseudomonas populations. The hypothesis was that hypermutability would be rare in early and in environmental but in contrast would explain the relatively recent emergence of epidemic strains. It was found that 10/100 (10 %) of early isolates were strong or weak mutators, suggesting that the CF lung is not the only factor influencing the existence of mutators in this group of patients. Two weak mutators (6 %) were found in 32 environmental isolates. Only two of 15 (13 %) epidemic strains were hypermutable, and although closer analysis revealed this issue to be complex, on the whole the data suggested that the atypical characteristics of these highly transmissible strains cannot solely be explained by this phenomenon. The higher than predicted prevalence of mutators in early infection, and in environmental isolates, reinforces the importance of early and aggressive treatment for infection in CF.

Keyword(s): CF, cystic fibrosis
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2007-06-01
2019-11-16
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