RT Journal Article SR Electronic(1) A1 Bacon, Joanna A1 Dover, Lynn G A1 Hatch, Kim A A1 Zhang, Yi A1 Gomes, Jessica M A1 Kendall, Sharon A1 Wernisch, Lorenz A1 Stoker, Neil G A1 Butcher, Philip D A1 Besra, Gurdyal S A1 Marsh, Philip DYR 2007 T1 Lipid composition and transcriptional response of Mycobacterium tuberculosis grown under iron-limitation in continuous culture: identification of a novel wax ester JF Microbiology, VO 153 IS 5 SP 1435 OP 1444 DO https://doi.org/10.1099/mic.0.2006/004317-0 PB Microbiology Society, SN 1465-2080, AB The low level of available iron in vivo is a major obstacle for microbial pathogens and is a stimulus for the expression of virulence genes. In this study, Mycobacterium tuberculosis H37Rv was grown aerobically in the presence of limited iron availability in chemostat culture to determine the physiological response of the organism to iron-limitation. A previously unidentified wax ester accumulated under iron-limited growth, and changes in the abundance of triacylglycerol and menaquinone were also observed between iron-replete and iron-limited chemostat cultures. DNA microarray analysis revealed differential expression of genes involved in glycerolipid metabolism and isoprenoid quinone biosynthesis, providing some insight into the underlying genetic changes that correlate with cell-wall lipid profiles of M. tuberculosis growing in an iron-limited environment., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.2006/004317-0