1887

Abstract

The pathogenesis of infections is dependent upon expression of various virulence factors, which are under the control of multiple regulatory systems, including two-component regulatory systems and transcriptional regulators such as the SarA family of proteins. As a part of a continuing effort to understand the regulatory mechanisms that involve the SarA protein family, the regulation and physical characterization of transcription is described here. The gene, a member of the family of genes, was previously characterized and has been shown to regulate a large number of genes. The locus is composed of multiple overlapping transcripts as determined by primer extension and was proposed to encode an open reading frame of 133 residues. Transcription of was significantly increased in the mutant. Gel shift and transcriptional studies revealed that SarA could bind to the promoter region, probably acting as a repressor for transcription. The data indicate that the expression of transcription is significantly repressed only by SarA among the family of mutants tested at the post-exponential phase of growth.

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2007-05-01
2019-10-22
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