1887

Abstract

genomes contain genes homologous to the aerobactin receptor () and biosynthetic genes () as well as the ferric hydroxamate uptake system () of . However, is disrupted by a frameshift mutation. An strain carrying the cloned aerobactin region was unable to produce aerobactin, but could use the siderophore as an iron source. Repair of the frameshift mutation in did not allow aerobactin production in or . In contrast, a strain with a plasmid encoding the genes from or pColV-K30 did produce and secrete the siderophore. In addition, PB1, which encodes the locus without the specific frameshift mutation, also failed to produce aerobactin. The operon, encoding an ABC transporter for a range of hydroxamate siderophores, was able to complement a strain of with a transposon insertion in , allowing utilization of aerobactin and ferrichrome. KIM6, a strain deficient in the production of the siderophore yersiniabactin, was able to use both the ferrichrome and the aerobactin siderophores as a source of iron. Mutations in or the operon abolished the ability of KIM6 to use aerobactin. Mutations in the operon, but not in , affected the ability of KIM6 to use ferrichrome. This demonstrates that uses both ferrichrome and aerobactin, but has lost the ability to synthesize aerobactin.

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2007-07-01
2020-11-29
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