1887

Abstract

The genes of a small multidrug resistance (SMR) pump were identified as part of the pAO1-encoded nicotine regulon responsible for nicotine catabolism in . When [C]nicotine was added to the growth medium the bacteria exported the C-labelled end product of nicotine catabolism, methylamine. In the presence of the proton-motive force inhibitors 2,4-dinitrophenol (DNP), carbonyl cyanide -chlorophenylhydrazone (CCCP) or the proton ionophore nigericin, export of methylamine was inhibited and radioactivity accumulated inside the bacteria. Efflux of [C]nicotine-derived radioactivity from bacteria was also inhibited in a  :  strain with downregulated expression. Because of low amine oxidase levels in the  :  strain, --methylaminobutyrate, the methylamine precursor, accumulated. Complementation of this strain with the genes, carried on a plasmid, restored the efflux of nicotine breakdown products. Both NepA and NepB were required for full export activity, indicating that they form a two-component efflux pump. NepAB may function as a metabolic valve by exporting methylamine, the end product of nicotine catabolism, and, in conditions under which it accumulates, the intermediate --methylaminobutyrate.

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2007-05-01
2020-11-25
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